凝胶过滤后人尿中的免疫反应性促黄体生成素、促卵泡激素及其亚基

Immunoreactive luteinizing hormone, follicle stimulating hormone and their subunits in human urine following gel filtration.

作者信息

Beitins I Z, Derfel R L, O'Loughlin K, McArthur J W

出版信息

J Clin Endocrinol Metab. 1977 Jan;44(1):149-59. doi: 10.1210/jcem-44-1-149.

Abstract

Urine collected from postmenopausal women, normal men, women and children was prepared by vacuum dialysis and kaolin extraction prior to chromatography on Sephadex G-100. Specific radioimmunoassays for LH, alpha subunit, LHbeta, FSH and FSHbeta were utilized to define the elution patterns of the urinary filtrates, extracts, 2nd IRP-HMG and postmenopausal serum. The following findings were obtained: 1) FSH eluted as a distinct immunoreactive peak whereas LH eluted more diffusely, 2) free alpha subunit was present in all urinary preparations, the 2nd IRP-HMG and postmenopausal serum, 3) free FSHbeta but not LHbeta was detectable in postmenopausal urine, and 4) an LHbeta immunoreactive fragment with an approximate molecular weight of 5000 daltons was present in all urinary preparations and the 2nd IRP-HMG but not in postmenopausal serum.

摘要

从绝经后妇女、正常男性、女性和儿童收集的尿液，在Sephadex G - 100柱层析之前，通过真空透析和高岭土提取进行制备。利用促黄体生成素（LH）、α亚基、LHβ、促卵泡生成素（FSH）和FSHβ的特异性放射免疫测定法来确定尿液滤液、提取物、第二国际参考品-人绝经期促性腺激素（2nd IRP - HMG）和绝经后血清的洗脱模式。获得了以下结果：1）促卵泡生成素以一个明显的免疫反应峰洗脱，而促黄体生成素洗脱得较为弥散；2）游离α亚基存在于所有尿液制剂、第二国际参考品-人绝经期促性腺激素和绝经后血清中；3）在绝经后尿液中可检测到游离FSHβ，但未检测到游离LHβ；4）所有尿液制剂和第二国际参考品-人绝经期促性腺激素中存在一个分子量约为5000道尔顿的LHβ免疫反应片段，但绝经后血清中不存在。