Raid A, Fenske M, König A
Endokrinologie. 1979 Apr;73(2):157-61.
Urinary Lutropin (LH) and Follitropin (FSH) were precipitated with acetone and measured by specific radioimmunoassays. Recovery experiments with special regard to urinary pH values were done by adding 125I-labelled LH and FSH to unprocessed urine. At pH 4.5 68.6% of LH and 66.1% of FSH were recovered. Increasing pH values up to 6.0 resulted in a significant recovery loss of 125-I-LH, but not of 125I-FSH. Immunoreactive FSH concentrations decreased significantly 6 and 48 hours after storage began at room temperature. In healthy males and females we found mean LH and FSH concentrations in 24-h-urine which were similar to those reported by others: LH: males: 22.6 IU/24-h; cycling females: 17.8 IU/24-h; postmenopausal females: 49.1 IU/24-h; FSH: males 6.0 IU/24-h: cycling females: 15.0 IU/24-h; postmenopausal females: 48.5 IU/24-h. Because of their high sensitivity and practicability, we believe that these radioimmunoassays are clinically useful approaches in assessment of pituitary and gonadal disorders in human subjects.
用丙酮沉淀尿促黄体生成素（LH）和促卵泡生成素（FSH），并通过特异性放射免疫测定法进行测量。通过向未处理的尿液中添加125I标记的LH和FSH，进行了特别关注尿液pH值的回收率实验。在pH 4.5时，LH的回收率为68.6%，FSH的回收率为66.1%。将pH值提高到6.0会导致125I-LH的回收率显著降低，但125I-FSH的回收率没有降低。在室温下储存开始后6小时和48小时，免疫反应性FSH浓度显著下降。在健康男性和女性中，我们发现24小时尿液中的LH和FSH平均浓度与其他人报告的相似：LH：男性：22.6 IU/24小时；处于月经周期的女性：17.8 IU/24小时；绝经后女性：49.1 IU/24小时；FSH：男性6.0 IU/24小时；处于月经周期的女性：15.0 IU/24小时；绝经后女性：48.5 IU/24小时。由于其高灵敏度和实用性，我们认为这些放射免疫测定法是评估人类垂体和性腺疾病的临床有用方法。
