Penny R, Goldstein I P, Frasier D
J Clin Endocrinol Metab. 1976 Dec;43(6):1394-9. doi: 10.1210/jcem-43-6-1394.
FSH and LH in acetone precipitates of timed overnight urine collections were measured by radioimmunoassay. FSH and LH urinary excretion extrapolated to 24 h was determined in 147 boys 3-16 years and 20 normal men. FSH and LH (2nd IRP-HMG) progressively increased with age from 1.8 +/- 1.1 (FSH) and 2.1 +/- 1.2 (LH) IU/24 h for the 3-4 year age group to 8.6 +/- 3.2 (FSH) and 18.8 +/- 9.0 (LH) IU/24 h for the 15-16 year age group. FSH and LH excretion increased progressively with stage of sexual development. The integration of several hours of FSH and LH secretion and the accuracy, simplicity and convenience of collecting a single urine sample are advantages of the timed overnight urine collection which should make this technique extremely useful in the evaluation of gonadotropin function.
采用放射免疫分析法测定定时过夜尿液收集的丙酮沉淀物中的促卵泡激素（FSH）和促黄体生成素（LH）。对147名3至16岁的男孩和20名正常男性测定了推算至24小时的FSH和LH尿排泄量。FSH和LH（第二国际参考品-人绝经期促性腺激素）随年龄增长而逐渐增加，3至4岁年龄组为1.8±1.1（FSH）和2.1±1.2（LH）IU/24小时，15至16岁年龄组为8.6±3.2（FSH）和18.8±9.0（LH）IU/24小时。FSH和LH排泄量随性发育阶段逐渐增加。定时过夜尿液收集具有整合数小时FSH和LH分泌以及收集单一尿液样本的准确性、简便性和便利性等优点，这使得该技术在评估促性腺激素功能方面极为有用。
