Desai M A, Ghosh S, Crump P W, Benmakroha Y, Vadgama P M
Department of Medicine (Clinical Biochemistry), University of Manchester, Hope Hospital, Salford, United Kingdom.
Scand J Clin Lab Invest Suppl. 1993;214:53-60. doi: 10.3109/00365519309090679.
This study describes the use of discrete, highly selective covering inner membranes in amperometric enzyme electrodes for direct biofluid analysis. Such inner membranes when interposed between the enzyme layer and the electrode working surface has been found to be both selective, as well as biocompatible. Cellulose acetate or detergent (Triton X-100 and Tween 80) modified cellulose acetate and polyethersulphone polymeric membranes, were both found to be highly selective against electroactive solutes normally present in biofluids. The inclusion of such inner perm-selective membranes greatly enhanced the biocompatibility of the enzyme electrode and reduced the passivation of the working electrode on exposure to whole blood or serum. The study indicates that electrode drift in biological solutions may also be due to passivation of the working electrode by small diffusible surface active species.
本研究描述了在用于直接生物流体分析的安培酶电极中使用离散的、高选择性覆盖内膜的情况。已发现，当这种内膜置于酶层和电极工作表面之间时，它既具有选择性，又具有生物相容性。醋酸纤维素或经洗涤剂（吐温X - 100和吐温80）改性的醋酸纤维素以及聚醚砜聚合物膜，均对生物流体中通常存在的电活性溶质具有高度选择性。包含这种内部选择透过性膜极大地增强了酶电极的生物相容性，并减少了工作电极在暴露于全血或血清时的钝化现象。该研究表明，生物溶液中的电极漂移也可能是由于工作电极被小的可扩散表面活性物质钝化所致。
