Portal I, Clerc T, Sbarra V, Portugal H, Pauli A M, Lafont H, Tuchweber B, Yousef I, Chanussot F
Department of Nutrition, University of Montreal, Canada.
Am J Physiol. 1993 Jun;264(6 Pt 1):G1052-6. doi: 10.1152/ajpgi.1993.264.6.G1052.
The purpose of this work was to evaluate biliary phosphatidylcholine (PC) secretion after intravenous infusion of high density lipoprotein (HDL)-[3H]phosphatidylcholine (HDL-[3H]PC) in rats and to study the effect of infusion of dehydrocholic and cholic acids, which, respectively, inhibit and stimulate biliary secretion of PC. The data obtained in this study showed that, in the basal state, HDL-PC accounted for 38% of biliary PC. Dehydrocholic acid infusion caused only a "residual" secretion of HDL-PC in the bile; however, cholic acid infusion stimulated the secretion of HDL-PC as well as PC from intrahepatic microsomes. The low level of radioactivity of HDL-PC in intrahepatic compartments suggests that HDL-PC taken up by the liver is predestined for the bile secretion. The correlation between the kinetics of bile secretion of HDL-cholesterol and HDL-[3H]PC suggests the importance of HDL-PC in reverse transport of cholesterol to the liver and its transport to the bile. The differences between the effects of dehydrocholic acid and cholic acid infusions can be explained by the differences in bile salts binding to the surface of HDL.
本研究的目的是评估大鼠静脉输注高密度脂蛋白（HDL）-[3H]磷脂酰胆碱（HDL-[3H]PC）后胆汁中磷脂酰胆碱（PC）的分泌情况，并研究脱氢胆酸和胆酸输注的影响，这两种物质分别抑制和刺激PC的胆汁分泌。本研究获得的数据表明，在基础状态下，HDL-PC占胆汁PC的38%。输注脱氢胆酸仅导致胆汁中HDL-PC的“残余”分泌；然而，输注胆酸刺激了HDL-PC以及肝内微粒体中PC的分泌。肝内隔室中HDL-PC的放射性水平较低，这表明肝脏摄取的HDL-PC注定要分泌到胆汁中。HDL-胆固醇和HDL-[3H]PC胆汁分泌动力学之间的相关性表明，HDL-PC在胆固醇逆向转运至肝脏及其向胆汁转运过程中具有重要作用。脱氢胆酸和胆酸输注效果的差异可以通过胆汁盐与HDL表面结合的差异来解释。
