来自假单胞菌属KWI-56的酯酶基因的克隆与序列分析
Cloning and sequence analysis of an esterase gene from Pseudomonas sp. KWI-56.
作者信息
Shimada Y, Nagao T, Sugihara A, Iizumi T, Yui T, Nakamura K, Fukase T, Tominaga Y
机构信息
Osaka Municipal Technical Research Institute, Japan.
出版信息
Biochim Biophys Acta. 1993 Jul 18;1174(1):79-82. doi: 10.1016/0167-4781(93)90095-u.
The gene encoding an esterase from Pseudomonas sp. KWI-56 was cloned and sequenced. The nucleotide sequence contained an open reading frame encoding a polypeptide comprising 262 amino acids, whose molecular weight agreed well with the value obtained by SDS-PAGE. Comparison of the amino acid sequence with those of the other homologous enzymes suggested that Ser-92 and His-24l might be included in the catalytic triad.
克隆并测定了来自假单胞菌属KWI-56的一种酯酶的编码基因。核苷酸序列包含一个开放阅读框，其编码一个由262个氨基酸组成的多肽，该多肽的分子量与通过SDS-PAGE获得的值非常吻合。将该氨基酸序列与其他同源酶的氨基酸序列进行比较表明，Ser-92和His-241可能包含在催化三联体中。
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