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产柔红霉素链霉菌中控制柔红霉素生物合成早期和晚期步骤的基因簇的功能表征及转录分析。

Functional characterization and transcriptional analysis of a gene cluster governing early and late steps in daunorubicin biosynthesis in Streptomyces peucetius.

作者信息

Madduri K, Hutchinson C R

机构信息

School of Pharmacy, University of Wisconsin, Madison 53706, USA.

出版信息

J Bacteriol. 1995 Jul;177(13):3879-84. doi: 10.1128/jb.177.13.3879-3884.1995.

DOI:10.1128/jb.177.13.3879-3884.1995
PMID:7601857
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC177111/
Abstract

Sequence analysis of the Streptomyces peucetius daunorubicin biosynthetic gene cluster revealed a partial (dnrQ) and two complete (dnrD and dnrP) open reading frames flanking dnrK. Bioconversion experiments showed that DnrD converts aklanonic acid methylester to aklaviketone and that DnrC is a methyltransferase that converts aklanonic acid to aklanonic acid methylester. The deduced dnrP gene product, homologous to known esterases, may catalyze the conversion of 10-carbomethoxy-13-deoxycarminomycin to its 10-carboxy derivative. The dnrKPQS genes may be transcribed as a polycistronic mRNA.

摘要

对天蓝色链霉菌柔红霉素生物合成基因簇的序列分析显示，在dnrK两侧有一个部分开放阅读框（dnrQ）和两个完整的开放阅读框（dnrD和dnrP）。生物转化实验表明，DnrD将阿克拉诺酸甲酯转化为阿克拉酮，并且DnrC是一种将阿克拉诺酸转化为阿克拉诺酸甲酯的甲基转移酶。推导的dnrP基因产物与已知酯酶同源，可能催化10-甲氧羰基-13-脱氧卡红菌素向其10-羧基衍生物的转化。dnrKPQS基因可能作为多顺反子mRNA进行转录。

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本文引用的文献

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Nucleotide sequence analysis of five putative Streptomyces griseus genes, one of which complements an early function in daunorubicin biosynthesis that is linked to a putative gene cluster involved in TDP-daunosamine formation.对五个推测的灰色链霉菌基因进行核苷酸序列分析，其中一个基因补充了柔红霉素生物合成中的早期功能，该功能与一个涉及TDP-柔红糖胺形成的推测基因簇相关。
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