Mattig F R, Drössigk U, Entzeroth R
Institut of Zoology, University of Bonn, Germany.
Appl Parasitol. 1993 May;34(2):139-42.
A rapid, simple and cheap method for the purification of Eimeria tenella sporozoites has been developed using commercially available filter paper (595 filter paper circles, Schleicher & Schuell, 3354 Dassel, Germany, order 311610). Yield and purity are of the same value as by purification with a leukopac column (Bontemps & Yvore 1974). The described method can be used for the purification of Eimeria tenella sporozoites under sterile conditions for subsequent in vitro cultivation.
已经开发出一种快速、简单且廉价的纯化柔嫩艾美耳球虫子孢子的方法，该方法使用市售滤纸（595号滤纸圆片，施莱歇尔&舒尔公司，德国达塞尔3354，订单号311610）。产量和纯度与使用白细胞滤柱纯化（邦temps&伊沃雷，1974年）时的值相同。所描述的方法可用于在无菌条件下纯化柔嫩艾美耳球虫子孢子，以便后续进行体外培养。
