Kravetz A N, Zakharova M V, Beletskaya I V, Sineva E V, Denjmuchametov M M, Petrov S I, Glatman L I, Solonin A S
Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region.
Gene. 1993 Jul 15;129(1):153-4. doi: 10.1016/0378-1119(93)90712-c.
The restriction endonucleases Eco1831I and EcoHI cleave before the first 5'-cytosine in the recognition sequence 5'-decreases CCSGG--3'/3'--GGSCC increases-5' (where S = G or C), generate 5-base 5' cohesive ends, and are encoded by homologous plasmids that are restricted in McrA+ hosts. Thus, they differ in their cleavage specificity from that of the BcnI isoschizomer, which cleaves after the second 5' cytosine.
限制性内切酶Eco1831I和EcoHI在识别序列5'-decreases CCSGG--3'/3'--GGSCC increases-5'（其中S = G或C）中第一个5'-胞嘧啶之前切割，产生5个碱基的5'粘性末端，并且由在McrA+宿主中受到限制的同源质粒编码。因此，它们的切割特异性与BcnI同裂酶不同，BcnI同裂酶在第二个5'胞嘧啶之后切割。
