Lunnen K D, Morgan R D, Timan C J, Krzycki J A, Reeve J N, Wilson G G
New England Biolabs, Inc., Beverly, MA 01915.
Gene. 1989 Apr 15;77(1):11-9. doi: 10.1016/0378-1119(89)90354-5.
R.MwoI, a type-II restriction enzyme with the new specificity 5'-GCN7GC-3', was found in extracts of the thermophilic archaebacterium, Methanobacterium wolfei. R.MwoI cleaves duplex DNA producing fragments with 3-nt, 3'-terminal extensions, thus: GCN5/N2GC. The genes coding for the MwoI restriction and modification enzymes were cloned into Escherichia coli on the plasmid vector pBR322. The clones synthesize a low level of R.MwoI endonuclease. The plasmids display incomplete MwoI-specific modification, suggesting that the clones synthesize a low level of the M.MwoI methyltransferase, too.
