Chee H, Romsos D R, Leveille G A
J Nutr. 1977 Jan;107(1):112-9. doi: 10.1093/jn/107.1.112.
The influence of (-)-hydroxycitrate on in vitro rates of fatty acid synthesis in chicken and rat liver and in rat adipose tissue was investigated. Following acute and chronic administration of (-)-hydroxycitrate to chickens and rats, changes in rates of fatty acid synthesis in vivo and in lipogenic enzyme activities were also determined. Addition of 1 mM (-)-hydroxycitrate to a buffer containing 10 mM glucose and liver slices from chickens or rats depressed the rate of 3H2O incorporation into fatty acids. Acute administration of (-)-hydroxycitrate to chickens and rats also depressed in vivo rates of fatty acid synthesis in the livers of these species. Chickens appeared to be more sensitive to the inhibitory effects of (-)-hydroxycitrate than did rats. (-)-Hydroxycitrate was incorporated into the diets fed to the chickens and rats. Hepatic citrate cleavage enzyme activities were increased several fold in livers of chickens and rats fed (-)-hydroxycitrate. However, the rates of fatty acid synthesis in chicken and rat livers were depressed when expressed per g liver. Livers of chickens fed (-)-hydroxycitrate for 14 days were enlarged. Dietary (-)-hydroxycitrate did not depress rates of fatty acid synthesis per liver in these birds. Consumption of (-)-hydroxycitrate did not alter liver weight in rats. In vivo rates of fatty acid synthesis in rat adipose tissue were not influenced by consumption of a diet containing 52.6 nmoles (-)-hydroxycitrate/kg diet. Plasma triglyceride levels were increased two-fold in chickens, but unchanged in rats, fed (-)-hydroxycitrate for 2 to 3 weeks. There are species-specific as well as organ-specific responses to (-)-hydroxycitrate.
研究了（-）-羟基柠檬酸对鸡和大鼠肝脏以及大鼠脂肪组织中脂肪酸合成体外速率的影响。在对鸡和大鼠急性和慢性给予（-）-羟基柠檬酸后，还测定了体内脂肪酸合成速率和生脂酶活性的变化。向含有10 mM葡萄糖和鸡或大鼠肝脏切片的缓冲液中添加1 mM（-）-羟基柠檬酸，可降低3H2O掺入脂肪酸的速率。对鸡和大鼠急性给予（-）-羟基柠檬酸也会降低这些物种肝脏中脂肪酸合成的体内速率。鸡似乎比大鼠对（-）-羟基柠檬酸的抑制作用更敏感。（-）-羟基柠檬酸被添加到喂给鸡和大鼠的饲料中。在喂食（-）-羟基柠檬酸的鸡和大鼠肝脏中，柠檬酸裂解酶活性增加了几倍。然而，以每克肝脏计算时，鸡和大鼠肝脏中的脂肪酸合成速率却降低了。喂食（-）-羟基柠檬酸14天的鸡肝脏肿大。在这些鸡中，饲料中的（-）-羟基柠檬酸并未降低每个肝脏的脂肪酸合成速率。食用（-）-羟基柠檬酸不会改变大鼠的肝脏重量。食用含有52.6纳摩尔/千克饲料的（-）-羟基柠檬酸的饲料，对大鼠脂肪组织中脂肪酸合成速率没有影响。喂食（-）-羟基柠檬酸2至3周后，鸡的血浆甘油三酯水平增加了两倍，但大鼠的血浆甘油三酯水平没有变化。对（-）-羟基柠檬酸存在物种特异性和器官特异性反应。
