Schneeman B O, Chang I, Smith L B, Lyman R L
J Nutr. 1977 Feb;107(2):281-8. doi: 10.1093/jn/107.2.281.
Pancreatic enzyme output in response to various purified diets was studied in rats surgically prepared so that pancreatic secretion could be continuously collected, assayed and returned to the intestine. Intraduodenal infusion of phenylalanine and tryptophan alone did not stimulate secretion. Diets containing phenylalanine, tryptophan, a mixture of amino acids, or hydroxyzed casein, fed intragastrically, evoked a small pancreatic response that was similar to the response to a protein-free diet. intragastric infusion of a diet containing 18% casein stimulated a large initial secretion of enzyme that remained elevated throughout the 5.5 hour experiment. Addition of soybean trypsin inhibitor (SBTI) to the diet increased the pancreatic response over that due to dietary casein alone. When pancreatic juice was diverted from the intestine, the large pancreatic responses to casein or to casein + SBTI were greatly reduced and the response was similar to that of the protein-free diet.
在通过手术制备的大鼠中研究了胰腺酶对各种纯化饮食的分泌反应，这样可以连续收集、检测胰腺分泌物并将其回输到肠道。单独十二指肠内输注苯丙氨酸和色氨酸不会刺激分泌。胃内饲喂含有苯丙氨酸、色氨酸、氨基酸混合物或水解酪蛋白的饮食，会引起较小的胰腺反应，这与对无蛋白饮食的反应相似。胃内输注含有18%酪蛋白的饮食会刺激酶的大量初始分泌，在整个5.5小时的实验过程中这种分泌一直保持在较高水平。在饮食中添加大豆胰蛋白酶抑制剂（SBTI）会使胰腺反应比仅由饮食酪蛋白引起的反应有所增加。当胰液从肠道分流时，对酪蛋白或酪蛋白+SBTI的大量胰腺反应会大大降低，且该反应与无蛋白饮食的反应相似。
