Karam S M, Leblond C P
Department of Anatomy, McGill University, Montreal, Canada.
Anat Rec. 1993 Jun;236(2):333-40. doi: 10.1002/ar.1092360206.
Entero-endocrine cells and the rare cells named caveolated or brush cells have been examined in light microscopic radioautographs of the mouse corpus after various periods of continuous 3H-thymidine infusion. Moreover a search for immature forms and mitoses of these cells was undertaken in the electron microscope. Entero-endocrine cells are present in the four regions of the epithelial units, but their number is low in the pit, intermediate in the isthmus and neck, and high in the base. The labeling pattern after continuous 3H-thymidine infusion indicates that these cells are produced in the isthmus from undifferentiated granule-free cells presumed to be the stem cells of the epithelium, and may retain a limited ability to divide. A few of the newly formed entero-endocrine cells migrate to the pit, but the majority goes to the neck and, from there, to the base where they are present in relatively high numbers. Little information is available on the dynamics of caveolated cells. Since immature forms are present in the isthmus and mature ones in the other regions, it is concluded that they arise in the isthmus and migrate away in both directions. Finally, concluding remarks are presented on the kinetics of each one of the cell lineages described in this and the four previous articles.
在对小鼠胃体进行不同时间段的连续3H-胸腺嘧啶核苷灌注后，已在光学显微镜放射自显影片中对肠内分泌细胞以及名为空泡状细胞或刷状细胞的稀有细胞进行了检查。此外，还利用电子显微镜对这些细胞的未成熟形式和有丝分裂进行了研究。肠内分泌细胞存在于上皮单位的四个区域，但在隐窝中数量较少，在峡部和颈部数量中等，而在底部数量较多。连续灌注3H-胸腺嘧啶核苷后的标记模式表明，这些细胞是由推测为上皮干细胞的未分化无颗粒细胞在峡部产生的，并且可能保留有限的分裂能力。少数新形成的肠内分泌细胞迁移至隐窝，但大多数迁移至颈部，然后从颈部迁移至底部，在底部它们的数量相对较多。关于空泡状细胞的动态变化，目前所知甚少。由于未成熟形式存在于峡部，而成熟形式存在于其他区域，因此可以得出结论，它们起源于峡部并向两个方向迁移。最后，对本文以及之前四篇文章中描述的每种细胞谱系的动力学进行了总结。
