Shida T, Iwaori H, Arakawa M, Sekiguchi J
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Nagano, Japan.
Chem Pharm Bull (Tokyo). 1993 May;41(5):961-4. doi: 10.1248/cpb.41.961.
To elucidate the conformational properties of DNA with a 2-deoxyribosylformamide residue (dF), an oligodeoxyribonucleotide containing this abasic residue in a specific position of the nucleotide sequence was synthesized by the standard solid-phase phosphotriester method. Deprotection of the synthesized oligonucleotide was performed under routine alkaline and acidic conditions. The presence of a dF residue in the oligomer was confirmed by ion-spray mass spectrometry. A dF residue was found to affect considerably the stability of the DNA duplex, as determined from the melting behavior of the dF-containing duplex.
为阐明含有2-脱氧核糖基甲酰胺残基（dF）的DNA的构象性质，通过标准固相磷酸三酯法合成了在核苷酸序列特定位置含有该无碱基残基的寡脱氧核糖核苷酸。合成的寡核苷酸在常规碱性和酸性条件下进行脱保护。通过离子喷雾质谱法确认了低聚物中dF残基的存在。从含dF双链体的解链行为确定，dF残基对DNA双链体的稳定性有相当大的影响。
