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Effects of an abasic site on triple helix formation characterized by affinity cleaving.

作者信息

Horne D A, Dervan P B

机构信息

Arnold and Mabel Beckman Laboratory of Chemical Synthesis, California Institute of Technology, Pasadena 91125.

出版信息

Nucleic Acids Res. 1991 Sep 25;19(18):4963-5. doi: 10.1093/nar/19.18.4963.

DOI:10.1093/nar/19.18.4963
PMID:1923764
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC328797/
Abstract

The stability of triple helical complexes of pyrimidine oligodeoxyribonucleotides containing one abasic 1,2-dideoxy-D-ribose (phi) residue was examined by affinity cleaving. Within a pyrimidine third strand, the triplets phi.AT, phi.GC, phi.TA and phi.CG are significantly less stable than the triplets, T.AT, C+GC and G.TA. The decrease in binding produced by an abasic residue is similar to that observed with imperfectly matched natural base triplets, with phi.AT and phi.GC being less stable than phi.TA and phi.CG triplets for the sequences studied.

摘要

相似文献

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本文引用的文献

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Synthesis and biophysical studies of short oligodeoxynucleotides with novel modifications: a possible approach to the problem of mixed base oligodeoxynucleotide synthesis.具有新型修饰的短寡脱氧核苷酸的合成与生物物理研究：解决混合碱基寡脱氧核苷酸合成问题的一种可能方法。
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Sequence-specific cleavage of single-stranded DNA: oligodeoxynucleotide-EDTA X Fe(II).单链DNA的序列特异性切割：寡聚脱氧核苷酸-乙二胺四乙酸×亚铁离子（II）
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Sequence-specific binding and photocrosslinking of alpha and beta oligodeoxynucleotides to the major groove of DNA via triple-helix formation.
在通透完整细胞中对天然基因进行三链体靶向：趋化因子受体CCR5基因的共价修饰
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Nucleic Acids Res. 1996 Jul 1;24(13):2606-13. doi: 10.1093/nar/24.13.2606.
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Effect of abasic linker substitution on triplex formation, Sp1 binding, and specificity in an oligonucleotide targeted to the human Ha-ras promoter.无碱基连接子替换对靶向人Ha-ras启动子的寡核苷酸中三链体形成、Sp1结合及特异性的影响。
Nucleic Acids Res. 1994 May 25;22(10):1909-16. doi: 10.1093/nar/22.10.1909.
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Binding of T and T analogs to CG base pairs in antiparallel triplexes.T 及其类似物与反平行三链体中 CG 碱基对的结合。
Nucleic Acids Res. 1994 Aug 11;22(15):3233-40. doi: 10.1093/nar/22.15.3233.
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Triplex formation inhibits HER-2/neu transcription in vitro.三链体形成在体外抑制HER-2/neu转录。
J Clin Invest. 1993 Nov;92(5):2433-9. doi: 10.1172/JCI116850.
8
Azole substituted oligonucleotides promote antiparallel triplex formation at non-homopurine duplex targets.唑取代的寡核苷酸促进在非同源嘌呤双链靶标处形成反平行三链体。
Nucleic Acids Res. 1995 Feb 25;23(4):647-53. doi: 10.1093/nar/23.4.647.
9
Single strand targeted triplex formation: targeting purine-pyrimidine mixed sequences using abasic linkers.单链靶向三链体形成：使用无碱基接头靶向嘌呤-嘧啶混合序列。
Nucleic Acids Res. 1995 Nov 11;23(21):4510-7. doi: 10.1093/nar/23.21.4510.
10
Elucidation of the sequence-specific third-strand recognition of four Watson-Crick base pairs in a pyrimidine triple-helix motif: T.AT, C.GC, T.CG, and G.TA.嘧啶三链体基序中四种沃森-克里克碱基对（T.AT、C.GC、T.CG和G.TA）序列特异性第三链识别的阐释
Proc Natl Acad Sci U S A. 1992 May 1;89(9):3840-4. doi: 10.1073/pnas.89.9.3840.
α和β寡脱氧核苷酸通过三链螺旋形成与DNA大沟的序列特异性结合及光交联作用。
Proc Natl Acad Sci U S A. 1988 Mar;85(5):1349-53. doi: 10.1073/pnas.85.5.1349.
4
NMR studies of abasic sites in DNA duplexes: deoxyadenosine stacks into the helix opposite the cyclic analogue of 2-deoxyribose.DNA双链中无碱基位点的核磁共振研究：脱氧腺苷堆积到与2-脱氧核糖环类似物相对的螺旋中。
Biochemistry. 1988 Feb 9;27(3):924-31. doi: 10.1021/bi00403a013.
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Sequence-specific cleavage of double helical DNA by triple helix formation.通过三链螺旋形成实现双链螺旋DNA的序列特异性切割。
Science. 1987 Oct 30;238(4827):645-50. doi: 10.1126/science.3118463.
6
Influence of abasic and anucleosidic sites on the stability, conformation, and melting behavior of a DNA duplex: correlations of thermodynamic and structural data.无碱基和无核苷位点对DNA双链体稳定性、构象及解链行为的影响：热力学与结构数据的相关性
Proc Natl Acad Sci U S A. 1989 May;86(10):3614-8. doi: 10.1073/pnas.86.10.3614.
7
Recognition of thymine adenine.base pairs by guanine in a pyrimidine triple helix motif.嘧啶三螺旋基序中鸟嘌呤对胸腺嘧啶 - 腺嘌呤碱基对的识别
Science. 1989 Sep 1;245(4921):967-71. doi: 10.1126/science.2549639.
8
The abasic site as a challenge to DNA polymerase. A nuclear magnetic resonance study of G, C and T opposite a model abasic site.无碱基位点对DNA聚合酶构成的挑战。对模型无碱基位点对面的鸟嘌呤、胞嘧啶和胸腺嘧啶的核磁共振研究。
J Mol Biol. 1990 May 20;213(2):303-14. doi: 10.1016/S0022-2836(05)80192-5.
9
Thermodynamic characterization of the stability and the melting behavior of a DNA triplex: a spectroscopic and calorimetric study.DNA三链体稳定性及熔解行为的热力学表征：一项光谱与量热研究
Proc Natl Acad Sci U S A. 1990 Dec;87(23):9436-40. doi: 10.1073/pnas.87.23.9436.
10
Formation of intramolecular triplex in homopurine-homopyrimidine mirror repeats with point substitutions.含点突变的同型嘌呤-同型嘧啶镜像重复序列中分子内三链体的形成
Nucleic Acids Res. 1990 Nov 25;18(22):6621-4. doi: 10.1093/nar/18.22.6621.