Esteban M
J Virol. 1977 Feb;21(2):796-801. doi: 10.1128/JVI.21.2.796-801.1977.
The effect of rifampin on the replication of vaccinia DNA was studied in mouse L cells by a cytochemical techinque and by alkaline sucrose sedimentation analysis of newly synthesized viral DNA molecules. By the use of a fluorescent DNA-binding compound (Hoechst 33258), the sequential appearance, size, and location of the viral "factories" in rifampin-treated, virus-infected cells were found to be indistinguishable from those observed in untreated, infected cells. Sedimentation analysis in alkaline scurose gradients of the viral DNA molecules labeled in pulse-chase experiments showed that formation of small fragments, elongation into "intermediate"-sized molecules, and maturation into full-length viral DNA and, finally, into cross-linked viral DNA molecules occurred in the absence or presence of rifampin. The results support the view that the primary effect of the drug is related to assembly or morphogenesis.
通过细胞化学技术以及对新合成的病毒DNA分子进行碱性蔗糖沉降分析，在小鼠L细胞中研究了利福平对痘苗病毒DNA复制的影响。使用一种荧光DNA结合化合物（Hoechst 33258），发现利福平处理的病毒感染细胞中病毒“工厂”的相继出现、大小和位置与未处理的感染细胞中观察到的情况没有区别。对脉冲追踪实验中标记的病毒DNA分子在碱性蔗糖梯度中的沉降分析表明，无论有无利福平，都会形成小片段、延伸为“中等”大小的分子、成熟为全长病毒DNA，最终形成交联的病毒DNA分子。这些结果支持了该药物的主要作用与组装或形态发生有关的观点。
