Kerns J M, Danielsen N, Holmquist B, Kanje M, Lundborg G
Department of Anatomy, Rush Presbyterian St. Luke's Medical Center, Chicago, Illinois.
Exp Neurol. 1993 Jul;122(1):28-36. doi: 10.1006/exnr.1993.1104.
Nerve regeneration through predegenerated (PNG) or fresh (FNG) autografts in either fresh or delayed recipient nerve beds were studied in the rat sciatic nerve. Grafts 10 mm in length were excised either immediately or following a 7-day period of predegeneration. They were sutured into gaps on the contralateral side which were either freshly made or had been made 7 days previously. The early recovery (10 days at 2-day intervals) was evaluated by the sensory pinch test to measure the rate of regeneration and the delay period. The PNG group had an improvement in axonal regeneration as evidenced by a reduced delay period, a reduced number of regeneration failures, and less variability in regeneration distances compared to the FNG group. Conditioning the host site blocked some of the delay reduction in the PNG group, but had no effect on regeneration rate or delay in the FNG group. The presence of axons in the graft was confirmed by immunocytochemistry for neurofilament protein and by electron microscopy. The results suggest that proliferated cells, primarily Schwann cells, promote regeneration through the suture line and graft into the distal segment.
在大鼠坐骨神经中，研究了通过预变性（PNG）或新鲜（FNG）自体移植物在新鲜或延迟受体神经床中的神经再生情况。长度为10毫米的移植物在预变性7天期间后或立即切除。将它们缝合到对侧的间隙中，这些间隙要么是新形成的，要么是7天前形成的。通过感觉捏压试验评估早期恢复情况（在2天间隔的10天内），以测量再生速率和延迟期。与FNG组相比，PNG组的轴突再生有所改善，表现为延迟期缩短、再生失败数量减少以及再生距离的变异性降低。对宿主部位进行预处理可阻断PNG组中部分延迟期的缩短，但对FNG组的再生速率或延迟期没有影响。通过神经丝蛋白免疫细胞化学和电子显微镜证实了移植物中轴突的存在。结果表明，增殖细胞（主要是雪旺细胞）促进再生通过缝线并长入远端节段。
