Suppr超能文献

新型抗岩藻糖基化抗原单克隆抗体YB-2的研制、特性及其在结直肠癌免疫组织化学诊断中的应用

Development and characterization of a novel anti-fucosylated antigen monoclonal antibody YB-2 and its usefulness in the immunohistochemical diagnosis of colorectal cancer.

作者信息

Yazawa S, Akamatsu S, Tachikawa T, Naito H, Nakamura J, Asao T, Nagamachi Y, Nakajima T, Shin S, Chia D

机构信息

Department of Legal Medicine, Gunma University School of Medicine, Maebashi.

出版信息

Jpn J Cancer Res. 1993 Jun;84(6):641-8. doi: 10.1111/j.1349-7006.1993.tb02024.x.

DOI:10.1111/j.1349-7006.1993.tb02024.x
PMID:8340252
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5919324/
Abstract

A novel monoclonal antibody, YB-2 was obtained after immunization of mice with fucosylated antigens isolated from human saliva. The antibody was demonstrated to react with Y (Fuc alpha 1-->2Gal-beta 1-->4[Fuc alpha 1-->3]GlcNAc beta),Leb (Fuc alpha 1-->2Gal beta 1-->3[Fuc alpha 1-->4]GlcNAc beta) and H type 2 (Fuc alpha 1-->2Gal-beta 1-->4GlcNAc beta) antigens, but not with H type 1 (Fuc alpha 1-->2Gal beta 1-->3GlcNAc beta), Lea (Gal beta 1-->3[Fuc alpha 1-->4]GlcNAc beta), X (Gal beta 1-->4[Fuc alpha 1-->3]GlcNAc beta) or with non-fucosylated antigens. Inhibition assays of YB-2 antibody with such reactive antigens showed that YB-2 antibody preferentially reacted with Y antigen. Formalin-fixed and paraffin-embedded sections prepared from normal and malignant colorectal tissues were examined immunohistochemically with YB-2. The positive rates of staining with YB-2 antibody were 88.6% in malignant and 12.0% in normal tissues. The expression of fucosylated antigens detected by YB-2 antibody seemed to be correlated with survival among patients with primary colorectal cancer. Therefore, YB-2 antibody could be useful as an immunochemical tool for diagnosis and evaluation of the prognosis of colorectal cancer.

摘要

用从人唾液中分离出的岩藻糖基化抗原免疫小鼠后，获得了一种新型单克隆抗体YB - 2。已证明该抗体可与Y（岩藻糖α1→2半乳糖-β1→4[岩藻糖α1→3]N - 乙酰葡糖胺β）、Leb（岩藻糖α1→2半乳糖β1→3[岩藻糖α1→4]N - 乙酰葡糖胺β）和H2型（岩藻糖α1→2半乳糖-β1→4N - 乙酰葡糖胺β）抗原发生反应，但不与H1型（岩藻糖α1→2半乳糖β1→3N - 乙酰葡糖胺β）、Lea（半乳糖β1→3[岩藻糖α1→4]N - 乙酰葡糖胺β）、X（半乳糖β1→4[岩藻糖α1→3]N - 乙酰葡糖胺β）或非岩藻糖基化抗原发生反应。用此类反应性抗原对YB - 2抗体进行抑制试验表明，YB - 2抗体优先与Y抗原发生反应。用YB - 2对从正常和恶性结直肠组织制备的福尔马林固定石蜡包埋切片进行免疫组织化学检查。YB - 2抗体染色的阳性率在恶性组织中为88.6%，在正常组织中为12.0%。YB - 2抗体检测到的岩藻糖基化抗原的表达似乎与原发性结直肠癌患者的生存率相关。因此，YB - 2抗体可作为一种免疫化学工具用于结直肠癌的诊断和预后评估。

相似文献

1
Development and characterization of a novel anti-fucosylated antigen monoclonal antibody YB-2 and its usefulness in the immunohistochemical diagnosis of colorectal cancer.新型抗岩藻糖基化抗原单克隆抗体YB-2的研制、特性及其在结直肠癌免疫组织化学诊断中的应用
Jpn J Cancer Res. 1993 Jun;84(6):641-8. doi: 10.1111/j.1349-7006.1993.tb02024.x.
2
Immunochemical characterization of anti-H monoclonal antibodies obtained from a mouse immunized with human saliva.从用人唾液免疫的小鼠中获得的抗-H单克隆抗体的免疫化学特性分析。
J Immunol Methods. 1993 Feb 26;159(1-2):261-7. doi: 10.1016/0022-1759(93)90165-4.
3
The recognition of cancer-associated fucosylated antigens in colorectal cancer by a novel monoclonal antibody, YB-2.新型单克隆抗体YB-2对结直肠癌中癌症相关岩藻糖基化抗原的识别
Surg Today. 1994;24(4):382-4. doi: 10.1007/BF02348574.
4
The monoclonal antibody directed to difucosylated type 2 chain (Fuc alpha 1 leads to 2Gal beta 1 leads to 4[Fuc alpha 1 leads to 3]GlcNAc; Y Determinant).针对二岩藻糖基化2型链（岩藻糖α1→2半乳糖β1→4[岩藻糖α1→3]N-乙酰葡糖胺；Y决定簇）的单克隆抗体
J Biol Chem. 1983 Oct 10;258(19):11793-7.
5
Biosynthetic pathways for the Leb and Y glycolipids in the gastric carcinoma cell line KATO III as analyzed by a novel assay.通过一种新型检测方法分析胃癌细胞系KATO III中Leb和Y糖脂的生物合成途径。
Biochem Biophys Res Commun. 1988 Feb 29;151(1):100-8. doi: 10.1016/0006-291x(88)90564-5.
6
Biosynthesis of Lewis fucolipid antigens in human colorectal carcinoma cells. Partial characterization of LcOse4Cer and H-1 fucolipid fucosyl transferase acceptor activities.人结肠癌细胞中Lewis岩藻糖脂抗原的生物合成。LcOse4Cer和H-1岩藻糖脂岩藻糖基转移酶受体活性的部分表征。
Eur J Biochem. 1987 Oct 1;168(1):209-17. doi: 10.1111/j.1432-1033.1987.tb13407.x.
7
Y and blood group B type 2 glycolipid antigens accumulate in a human gastric carcinoma cell line as detected by monoclonal antibody. Isolation and characterization by mass spectrometry and NMR spectroscopy.
J Biol Chem. 1987 Jan 5;262(1):372-9.
8
Aberrant alpha 1-->2fucosyltransferases found in human colorectal carcinoma involved in the accumulation of Leb and Y antigens in colorectal tumors.在人类结直肠癌中发现的异常α1→2岩藻糖基转移酶与结直肠肿瘤中Leb和Y抗原的积累有关。
Jpn J Cancer Res. 1993 Sep;84(9):989-95. doi: 10.1111/j.1349-7006.1993.tb00190.x.
9
Monosialogangliosides of human myelogenous leukemia HL60 cells and normal human leukocytes. 2. Characterization of E-selectin binding fractions, and structural requirements for physiological binding to E-selectin.人髓性白血病HL60细胞和正常人白细胞的单唾液酸神经节苷脂。2. E-选择素结合组分的特性，以及与E-选择素生理性结合的结构要求。
Biochemistry. 1996 Jan 23;35(3):770-8. doi: 10.1021/bi952461g.
10
Glycosphingolipid carriers of carbohydrate antigens of human myeloid cells recognized by monoclonal antibodies.被单克隆抗体识别的人髓细胞碳水化合物抗原的糖鞘脂载体。
Biochim Biophys Acta. 1985 Jul 30;846(1):26-36. doi: 10.1016/0167-4889(85)90106-5.

引用本文的文献

1
Blood group substances as potential therapeutic agents for the prevention and treatment of infection with noroviruses proving novel binding patterns in human tissues.血型物质作为预防和治疗诺如病毒感染的潜在治疗剂，在人体组织中呈现出新的结合模式。
PLoS One. 2014 Feb 18;9(2):e89071. doi: 10.1371/journal.pone.0089071. eCollection 2014.
2
MAb L9E10 to blood group H2 antigen binds to colon cancer stem cells and inhibits tumor cell migration and invasion.针对血型H2抗原的单克隆抗体L9E10可与结肠癌干细胞结合，并抑制肿瘤细胞的迁移和侵袭。
Hybridoma (Larchmt). 2010 Aug;29(4):355-9. doi: 10.1089/hyb.2010.0020.
3
Expression of H type 1 antigen of ABO histo-blood group in normal colon and aberrant expressions of H type 2 and H type 3/4 antigens in colon cancer.ABO组织血型H1抗原在正常结肠中的表达及H2抗原和H3/4抗原在结肠癌中的异常表达。
Glycoconj J. 2000 May;17(5):331-8. doi: 10.1023/a:1007173722426.
4
A new method for assaying adhesion of cancer cells to the greater omentum and its application for evaluating anti-adhesion activities of chemically synthesized oligosaccharides.一种检测癌细胞与大网膜黏附的新方法及其在评估化学合成寡糖抗黏附活性中的应用。
Clin Exp Metastasis. 2000;18(1):37-43. doi: 10.1023/a:1026526829010.
5
The usefulness of anti-fucosylated antigen antibody YB-2 for diagnosis of hepatocellular carcinoma.抗岩藻糖基化抗原抗体YB-2在肝细胞癌诊断中的应用价值。
Glycoconj J. 1997 Jan;14(1):81-7. doi: 10.1023/a:1018517116368.
6
Elevation of an alpha(1,3)fucosyltransferase activity correlated with apoptosis in the human colon adenocarcinoma cell line, HT-29.α(1,3)岩藻糖基转移酶活性的升高与人类结肠腺癌细胞系HT-29中的细胞凋亡相关。
Glycoconj J. 1996 Dec;13(6):1021-9. doi: 10.1007/BF01053198.
7
Aberrant alpha 1-->2fucosyltransferases found in human colorectal carcinoma involved in the accumulation of Leb and Y antigens in colorectal tumors.在人类结直肠癌中发现的异常α1→2岩藻糖基转移酶与结直肠肿瘤中Leb和Y抗原的积累有关。
Jpn J Cancer Res. 1993 Sep;84(9):989-95. doi: 10.1111/j.1349-7006.1993.tb00190.x.
8
The recognition of cancer-associated fucosylated antigens in colorectal cancer by a novel monoclonal antibody, YB-2.新型单克隆抗体YB-2对结直肠癌中癌症相关岩藻糖基化抗原的识别
Surg Today. 1994;24(4):382-4. doi: 10.1007/BF02348574.

本文引用的文献

1
Sphingoglycolipids with Leb activity, and the co-presence of Lea-, Leb-glycolipids in human tumor tissue.
Biochim Biophys Acta. 1970 Feb 10;202(1):225-8. doi: 10.1016/0005-2760(70)90244-4.
2
Distribution of blood group antigens A, B, H, Lewisa, and Lewisb in human normal, fetal, and malignant colonic tissue.血型抗原A、B、H、Lewis a和Lewis b在人正常、胎儿及恶性结肠组织中的分布
Cancer Res. 1985 Sep;45(9):4499-511.
3
Use of monoclonal antibodies to sialylated Lewisx and sialylated Lewisa for serological tests of cancer.
Cancer Res. 1985 Jan;45(1):435-7.
4
Aberrant glycosylation in cancer cell membranes as focused on glycolipids: overview and perspectives.聚焦于糖脂的癌细胞膜异常糖基化：综述与展望。
Cancer Res. 1985 Jun;45(6):2405-14.
5
Differential expression of difucosyl type 2 chain (LeY) defined by monoclonal antibody AH6 in different locations of colonic epithelia, various histological types of colonic polyps, and adenocarcinomas.由单克隆抗体AH6所定义的二岩藻糖基2型链（LeY）在结肠上皮不同部位、各种组织学类型的结肠息肉及腺癌中的差异表达。
Cancer Res. 1986 May;46(5):2639-44.
6
Lewisx- and sialylated Lewisx-related antigen expression in human malignant and nonmalignant colonic tissues.Lewisx及唾液酸化Lewisx相关抗原在人恶性和非恶性结肠组织中的表达
Cancer Res. 1986 May;46(5):2627-32.
7
Expression of Lewisa, Lewisb, X, and Y blood group antigens in human colonic tumors and normal tissue and in human tumor-derived cell lines.Lewis a、Lewis b、X和Y血型抗原在人结肠肿瘤、正常组织及人肿瘤衍生细胞系中的表达。
Cancer Res. 1986 Mar;46(3):1553-61.
8
Cancer-associated elevation of alpha(1----3)-L-fucosyltransferase activity in human serum.
Cancer. 1988 Aug 1;62(3):516-20. doi: 10.1002/1097-0142(19880801)62:3<516::aid-cncr2820620313>3.0.co;2-4.
9
Expression of Ley antigen in human immunodeficiency virus-infected human T cell lines and in peripheral lymphocytes of patients with acquired immune deficiency syndrome (AIDS) and AIDS-related complex (ARC).莱伊抗原在人类免疫缺陷病毒感染的人类T细胞系以及获得性免疫缺陷综合征（艾滋病）和艾滋病相关综合征（ARC）患者外周淋巴细胞中的表达。
J Exp Med. 1988 Feb 1;167(2):323-31. doi: 10.1084/jem.167.2.323.
10
The presence of CA19-9 in serum and saliva from Lewis blood-group negative cancer patients.Lewis血型阴性癌症患者血清和唾液中CA19-9的存在情况。
Jpn J Cancer Res. 1988 Apr;79(4):538-43. doi: 10.1111/j.1349-7006.1988.tb01624.x.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型，支持多种主流文档格式。

立即体验