Nakamura J, Yazawa S, Hada T, Asao T, Naitoh H, Takenoshita S, Kosaka M, Akamatsu S, Tachikawa T, Nagamachi Y
First Department of Surgery, Gunma University School of Medicine, Maebashi, Japan.
Glycoconj J. 1997 Jan;14(1):81-7. doi: 10.1023/a:1018517116368.
Levels of fucosylated antigens in sera from patients with liver diseases were examined by a newly developed sandwich-type enzyme immuno assay with the aid of anti-fucosylated antigen antibody, YB-2 which reacts simultaneously with Y, Leb and H type 2 antigens. When the cut-off value was set arbitrarily at mean +3 SD values of normal, 30 (69.8%) of the 43 patients with HCC, 14 (53.8%) of the 26 patients with liver cirrhosis (LC) and 24 (45.3%) of the 53 patients with chronic hepatitis (CH) were found to be positive, whereas all of the 30 samples from healthy controls were negative. The levels of alpha-fetoprotein (AFP) and protein induced by vitamin K absence or antagonist-II (PIVKA-II) in HCC were not correlated with those of YB-2 antigens. The positive rates of the combination YB-2 and AFP assay and YB-2 and PIVKA-II assay in HCC were significantly higher (83.7 and 86.0%, respectively) than that of the AFP and PIVKA-II combination (65.1%) which had been reported to be the best combination up to this time.
借助抗岩藻糖基化抗原抗体YB-2，采用新开发的夹心型酶免疫分析法检测肝病患者血清中岩藻糖基化抗原的水平，该抗体可同时与Y、Leb和H2型抗原发生反应。当将临界值任意设定为正常均值加3个标准差时，43例肝癌患者中有30例（69.8%）、26例肝硬化患者中有14例（53.8%）、53例慢性肝炎患者中有24例（45.3%）呈阳性，而30份健康对照样本均为阴性。肝癌患者中α-甲胎蛋白（AFP）和维生素K缺乏或拮抗剂-II诱导蛋白（PIVKA-II）的水平与YB-2抗原水平无关。肝癌中YB-2与AFP联合检测以及YB-2与PIVKA-II联合检测的阳性率（分别为83.7%和86.0%）显著高于AFP与PIVKA-II联合检测的阳性率（65.1%），此前报道AFP与PIVKA-II联合检测是最佳组合。
