Girdler N M
Department of Oral and Maxillofacial Surgery, Guy's Hospital, London, UK.
Int J Oral Maxillofac Surg. 1993 Jun;22(3):178-84. doi: 10.1016/s0901-5027(05)80248-6.
This investigation aimed to identify the behaviour of primate mandibular condylar cartilage cells in an in vitro model. Cells were harvested from the mandibular condyles of 1-year-old marmosets and maintained in primary culture for up to 30 d. Cell proliferation, structure, and phenotype were assessed at regular intervals by phase-contrast microscopy, cytologic staining, and immunocytochemistry. Cell growth increased progressively, and cultures reached confluence after 22 d. Cells were initially small and round but later became enlarged and heterogeneous in shape. Polygonal and hypertrophic chondroblast-like cells dominated the mature cultures. Glycosaminoglycan synthesis increased as cultures aged. Type I collagen was produced by early cultures, whereas type II collagen predominated in mature confluent monolayers. It is suggested that this mixed population of cells underwent phenotypic changes in primary cell culture that closely resemble the normal in vivo maturation process.
本研究旨在确定灵长类动物下颌髁突软骨细胞在体外模型中的行为。从1岁狨猴的下颌髁突采集细胞，并在原代培养中维持长达30天。通过相差显微镜、细胞学染色和免疫细胞化学定期评估细胞增殖、结构和表型。细胞生长逐渐增加，培养物在22天后达到汇合。细胞最初小而圆，但后来变大且形状各异。多边形和肥大的成软骨细胞样细胞在成熟培养物中占主导地位。随着培养时间的延长，糖胺聚糖合成增加。早期培养物产生I型胶原，而在成熟汇合单层中II型胶原占主导。提示这种混合细胞群在原代细胞培养中经历了表型变化，与正常体内成熟过程非常相似。
