Salo L, Kantomaa T
Institute of Dentistry, University of Oulu, Finland.
Calcif Tissue Int. 1993 Jun;52(6):465-9. doi: 10.1007/BF00571338.
An experiment was designed to mimic orthopedic functional appliances in order to investigate the spatial and temporal characteristics of type II collagen secretion as a marker of cartilage maturation in the mandibular condyle of young rabbits. The position of the glenoid fossa in relation to the condyle was altered so that articulation now took place more posteriorly. Histological sections of the condyles of 15-, 20- and 30-day-old experimental and control animals were stained with toluidine blue and with an anti-type II collagen antibody. A widened progenitor cell layer was found posteriorly in the experimental condyles and a narrow layer was found anteriorly to the articulating region. The chondroblast layer was also widened posteriorly, whereas the hypertrophic cell zone was narrower; the opposite was seen anteriorly. The effect was marked in 15- and 20-day-old animals and weak in 30-day-old animals. Type II collagen stain and strong toluidine blue metachromasia were not observed in the progenitor cell zone until the chondroblasts had acquired a flattened, slightly hypertrophic morphology, which was found deeper in the experimental condyles than in the controls. This is interpreted as a slowing down of the differentiation of chondroblasts as a result of the force applied. The effect of masticatory function may also be explained in terms of delayed differentiation of chondroblasts and increased growth.
设计了一项实验来模拟矫形功能矫治器，以研究Ⅱ型胶原蛋白分泌的时空特征，作为幼兔下颌髁突软骨成熟的标志物。关节窝相对于髁突的位置发生改变，使得关节现在在更靠后的位置发生。对15日龄、20日龄和30日龄的实验动物和对照动物的髁突进行组织学切片，用甲苯胺蓝和抗Ⅱ型胶原蛋白抗体染色。在实验侧髁突的后部发现祖细胞层增宽，在关节区域前方发现狭窄层。成软骨细胞层在后部也增宽，而肥大细胞区变窄；在前方则观察到相反的情况。这种效应在15日龄和20日龄的动物中明显，在30日龄的动物中较弱。直到成软骨细胞获得扁平、略肥大的形态，才在祖细胞区观察到Ⅱ型胶原蛋白染色和强烈的甲苯胺蓝异染性，这种形态在实验侧髁突中比对照侧更深。这被解释为由于施加的力导致成软骨细胞分化减慢。咀嚼功能的影响也可以用成软骨细胞分化延迟和生长增加来解释。
