McNair A T, Dissous C, Duvaux-Miret O, Capron A
Centre d'Immunologie et de Biologie Parasitaire, Unité Mixte INSERM 167-CNRS 624, Institut Pasteur, Lille, France.
Gene. 1993 Feb 28;124(2):245-9. doi: 10.1016/0378-1119(93)90400-w.
The 28-kDa glutathione S-transferase (GST) of Schistosoma mansoni is considered a possible vaccine candidate for use against this medically important parasite. The gene encoding this molecule has been isolated from a lambda EMBL4 library by using the corresponding cDNA sequence as a probe. The gene contains four exons and is approximately 5.5 kb in length. Analysis of the 5' flanking region revealed the presence of a consensus AP-1 recognition site, 5'-TGACTCA, between nucleotides -231 and -225. Southern blot analysis suggested the presence of a single gene encoding the 28-kDa GST in the S. mansoni genome.
