Lockyer M J, Cooper H, Tite J, Rowan W, Crowe J S
Department of Cell Biology, Wellcome Research Laboratories, Beckenham, Kent.
Parasitology. 1993 Jun;106 ( Pt 5):451-7. doi: 10.1017/s0031182000076733.
A recombinant baculovirus-expressed hybrid protein containing epitopes for the C-terminal fragment of the Plasmodium falciparum precursor to the major merozoite surface antigens (PMMSA) and the tetrapeptide repeats of the circumsporozoite protein (CSP) was assessed for its immunogenicity. Murine MHC-II restriction of the antibody response to the CSP repeats was not overcome by the PMMSA component, the response to which showed no restriction. In an adjuvant trial the highest antibody titres in rabbits to both components of the hybrid were obtained using Freund's adjuvant. Lack of a boosting antibody response to the CSP repeats appeared to be linked to the conformation of the PMMSA component. Formulation of the hybrid protein into Iscoms gave antibody titres of only short duration to both components.
对一种重组杆状病毒表达的杂合蛋白进行了免疫原性评估，该杂合蛋白包含恶性疟原虫主要裂殖子表面抗原前体（PMMSA）C端片段的表位以及环子孢子蛋白（CSP）的四肽重复序列。针对CSP重复序列的抗体反应的小鼠MHC-II限制性未被PMMSA成分克服，对PMMSA成分的反应未显示出限制性。在一项佐剂试验中，使用弗氏佐剂在兔中获得了针对杂合蛋白两种成分的最高抗体滴度。对CSP重复序列缺乏增强抗体反应似乎与PMMSA成分的构象有关。将杂合蛋白制成免疫刺激复合物（Iscoms）后，对两种成分的抗体滴度仅持续较短时间。
