Mönig H, Konermann G
Institut für Biophysik und Strahlenbiologie, Universität Freiburg, Germany.
Strahlenther Onkol. 1993 Jul;169(7):418-26.
In investigations with mice the influence of an X-irradiation on the chemiluminescence yield of granulocytes from the peripheral blood was measured. The measurements were carried out with diluted freshly drawn blood by adding luminol as chemiluminogenic substance. The granulocytes were stimulated by zymosan. The following dependencies were investigated: dose from 1 to 8 Gy and time twelve hours to 32 days after a whole-body irradiation. In general the chemiluminescence yields from blood of irradiated animals are reduced in dependence of dose compared with the findings in controls. In unirradiated animals (controls) there exists a good correlation between the numbers of granulocytes and the chemiluminescence. This correlation is diminished after an irradiation. For the fourth day after a whole-body irradiation the chemiluminescence shows an exponential dose dependence. If chemiluminescence counts are related to the number of granulocytes (relative chemiluminescence) an increased chemiluminescence per single granulocyte is recorded up to the eighth day after an irradiation with 1 Gy. It may be assumed that subpopulations of granulocytes with enhanced ability to form oxidative metabolic products are responsible for this effect.
在对小鼠的研究中，测量了X射线辐射对外周血粒细胞化学发光产量的影响。测量是通过在新鲜抽取的稀释血液中加入鲁米诺作为化学发光物质来进行的。粒细胞用酵母聚糖刺激。研究了以下相关性：全身照射后剂量从1到8 Gy以及时间从12小时到32天。一般来说，与对照组的结果相比，受辐照动物血液的化学发光产量随剂量降低。在未受辐照的动物（对照组）中，粒细胞数量与化学发光之间存在良好的相关性。辐照后这种相关性减弱。全身照射后第四天，化学发光呈现指数剂量依赖性。如果将化学发光计数与粒细胞数量相关联（相对化学发光），在用1 Gy照射后直至第八天，单个粒细胞的化学发光增加。可以假设具有增强的氧化代谢产物形成能力的粒细胞亚群是造成这种效应的原因。
