用化学发光法测定小鼠血液中吞噬细胞亚致死剂量X射线照射的早期效应及胱胺的影响。

The early effect of sublethal X-irradiation of phagocytic cells in mouse blood and the influence of cystamine as measured by chemiluminescence.

作者信息

Hovestadt I, Ernst M, Mönig H, Fischer H

出版信息

Int J Radiat Biol Relat Stud Phys Chem Med. 1983 Dec;44(6):563-73. doi: 10.1080/09553008314551631.

Abstract

The metabolic burst accompanying phagocytosis of granulocytes (PMN) leads to the generation of activated oxygen species such as O-2, H2O2, 1O2 and OH; which give rise to chemiluminescence (CL) in the presence of luminol. Reliable CL-measurements of stimulated PMN can be carried out in freshly drawn mouse blood, when photon counts are related to the number of PMN. Effects of low dose total body X-irradiation were studied using C57B1/6 mice. It was found that 24 and 48 hours after irradiation (0.24-0.95 Gy) CL of whole blood was slightly decreased. If however CL-counts were related to the number of PMN, an enhanced CL per single granulocyte was recorded. The administration of cystamine leads to an immune stimulating effect of unirradiated animals. In animals, who received 0.95 Gy a distinct radioprotective effect of cystamine can be observed.

摘要

粒细胞（PMN）吞噬作用伴随的代谢爆发会导致活性氧如超氧阴离子（O₂⁻）、过氧化氢（H₂O₂）、单线态氧（¹O₂）和羟自由基（OH）的产生；在鲁米诺存在的情况下，这些活性氧会引发化学发光（CL）。当光子计数与PMN数量相关时，可在新鲜采集的小鼠血液中对受刺激的PMN进行可靠的CL测量。使用C57B1/6小鼠研究了低剂量全身X射线照射的影响。结果发现，照射（0.24 - 0.95 Gy）后24小时和48小时，全血的CL略有下降。然而，如果CL计数与PMN数量相关，则单个粒细胞的CL增强。给予半胱胺会对未受照射的动物产生免疫刺激作用。在接受0.95 Gy照射的动物中，可以观察到半胱胺具有明显的辐射防护作用。