Reis K J, Chachowski R, Cupido A, Davies D, Jakway J, Setcavage T M
Ortho Diagnostic Systems Inc., Raritan, NJ.
Transfusion. 1993 Aug;33(8):639-43. doi: 10.1046/j.1537-2995.1993.33893342744.x.
A new system for typing and screening blood, based on the sieving effect of glass bead microparticles, has been developed. The test is performed in a microcolumn in which the red cell agglutinates are trapped in the glass bead matrix during centrifugation, and unagglutinated cells form a pellet at the bottom of the column. Anti-human globulin reagents were incorporated in the diluent and the new test system, column agglutination technology, was compared to conventional tube tests and low-ionic-strength method. Sera and plasmas (228 samples) were screened for red cell antibodies with two anti-human globulin reagents: one containing only anti-IgG and the other containing both anti-IgG and anti-C3b, -C3d. After initial testing, there was 94-percent agreement between column agglutination technology and tube tests, and after repeat testing, there was 97-percent agreement. The column agglutination technology anti-human globulin test eliminates the need to wash red cells, which decreases the overall test time. The test is easy to perform, and the results are more objective than those with tube and microplate methods.
一种基于玻璃珠微粒筛选效应的新型血型鉴定与筛查系统已被开发出来。该检测在微柱中进行，离心过程中红细胞凝集物被困在玻璃珠基质中，未凝集的细胞在柱底形成沉淀。抗人球蛋白试剂被添加到稀释液中，并将这种新的检测系统——柱凝集技术，与传统试管检测法和低离子强度法进行比较。使用两种抗人球蛋白试剂对血清和血浆（228份样本）进行红细胞抗体筛查：一种仅含抗IgG，另一种同时含抗IgG和抗C3b、C3d。初次检测后，柱凝集技术与试管检测法的符合率为94%，重复检测后，符合率为97%。柱凝集技术抗人球蛋白检测无需洗涤红细胞，从而缩短了整体检测时间。该检测操作简便，结果比试管法和微孔板法更客观。
