Lamy B, Tissot C, Heyd C, Lamy C
Regional Blood Transfusion Center, Besançon.
Transfus Clin Biol. 1994;1(2):121-7. doi: 10.1016/s1246-7820(94)80007-3.
A new system for irregular antibody screening was described in 1993 by Reis K.J. This test is performed in a microcolumn prefilled with glass microbeads in suspension in a neutral or Anti Human Globulin isotonic solution. When the red cells are sensitized they are trapped by the microbead suspension during column centrifugation. 21365 irregular antibody screenings were performed with this Column Agglutination Technology (CAT) and the results were compared to those obtained with conventional manual tests. The CAT was more efficient than the manual tests. The number of positive samples containing specific antibodies was higher with CAT tests (924 samples) than with manual tests (802 samples). The CAT is easy to perform and the elimination of washing steps decreases the overall test time. It allows the use of this test for pre-transfusion compatibility testing particularly in emergency transfusion cases. The red cell age and more generally the red cell storage conditions seem to be an influential parameter on the percentage of unspecific reactions. In this study the unspecific reaction rate was low but we used only reagent red cells prepared every day. This new technology may be considered as an alternative technology to the Gel Test in Blood Transfusion security.
1993年，里斯·K·J描述了一种新的不规则抗体筛查系统。该测试在一个微柱中进行，微柱预先填充有悬浮在中性或抗人球蛋白等渗溶液中的玻璃微珠。当红细胞被致敏时，在柱离心过程中它们会被微珠悬浮液捕获。使用这种柱凝集技术（CAT）进行了21365次不规则抗体筛查，并将结果与传统手工测试的结果进行了比较。CAT比手工测试更有效。含有特异性抗体的阳性样本数量，CAT测试（924个样本）比手工测试（802个样本）更多。CAT操作简便，省去洗涤步骤缩短了总体测试时间。它使得该测试可用于输血相容性预测试，尤其是在紧急输血情况下。红细胞的年龄以及更普遍的红细胞储存条件似乎是影响非特异性反应百分比的一个参数。在本研究中，非特异性反应率较低，但我们仅使用每天制备的试剂红细胞。在输血安全方面，这项新技术可被视为凝胶试验的替代技术。
