10(6) 使用新型白细胞去除滤器CF-2去除白细胞

10(6) Depletion of WBC with CF-2, a new leukocyte removal filter.

作者信息

Endo Y, Tani T, Yokota T, Tsutamoto Y, Kodama M

机构信息

First Department of Surgery, Shiga University of Medical Science, Japan.

出版信息

Am J Hematol. 1993 Sep;44(1):38-41. doi: 10.1002/ajh.2830440108.

DOI:10.1002/ajh.2830440108

PMID:8342563

Abstract

A new leukocyte removal filter, Nipro CF-2, was evaluated. A non-woven polyester filter, gravity flow device that does not require priming and rinsing after use was developed to remove white blood cells from 400 ml of whole blood or concentrates of 400 ml of whole blood. A flow cytometric technique was developed to measure extremely low WBC counts. To evaluate the efficiency of this filter, leukocyte counts were measured by flow cytometry using a Coulter EPIC-C cytometer. After filtration through a CF-2 filter, more than 99.9999% of WBCs were depleted from 6 of 17 samples. The mean value of WBC depletion was 99.9994%. The CF-2 filter achieved nearly a million-fold depletion of WBCs from whole blood concentrates.

摘要

对一种新型白细胞去除过滤器Nipro CF - 2进行了评估。开发了一种无需使用后预充和冲洗的重力流非织造聚酯过滤器，用于从400毫升全血或400毫升全血浓缩物中去除白细胞。开发了一种流式细胞术技术来测量极低的白细胞计数。为评估该过滤器的效率，使用库尔特EPIC - C细胞仪通过流式细胞术测量白细胞计数。通过CF - 2过滤器过滤后，17个样本中的6个样本白细胞减少率超过99.9999%。白细胞减少的平均值为99.9994%。CF - 2过滤器使全血浓缩物中的白细胞减少了近一百万倍。