Magnon K C, Jalbert M, Padhye A A
Department of Pathology, Baptist Memorial Hospital System, San Antonio, Tex.
Arch Pathol Lab Med. 1993 Aug;117(8):841-3.
Phialemonium obovatum was found to be the cause of nosocomial osteomyelitis in a 41-year-old man after sustaining a nonpenetrating injury to his lumbar and cervical region. Histologic examination of fragments of disk and bone from L3-4 hemilaminectomy showed multiple fragments of fibrocartilage with focal necrosis, chronic inflammation, and granulation tissue formation. Sections stained with Gomori's methenamine silver procedure showed multiple fungal elements in necrotic areas consisting of irregularly branched, hyaline septate hyphae having swollen cells, and occasional yeastlike cells. The use of Fontana-Masson silver stain showed the presence of melanin in cells walls and septa of the hyphae. Phialemonium obovatum was isolated when the ground tissue from disk and bone from L3-4 was cultured on biphasic brain-heart infusion medium. Colonies were moist, off-white to ochraceous with a characteristic green, diffusible pigment on the reverse side. The isolate grew well up to 40 degrees C. It formed characteristic adelophialides without conspicuous collarettes and basal septa and produced smooth, one-celled, hyaline, and obovate conidia.
在一名41岁男性腰椎和颈椎遭受非穿透性损伤后，发现卵形瓶霉是其医院获得性骨髓炎的病因。对L3 - 4半椎板切除术获取的椎间盘和骨碎片进行组织学检查，显示纤维软骨碎片存在局灶性坏死、慢性炎症和肉芽组织形成。经Gomori六胺银染色的切片在坏死区域显示多个真菌成分，由不规则分支、透明有隔菌丝组成，菌丝细胞肿胀，偶尔可见酵母样细胞。使用Fontana - Masson银染色显示菌丝细胞壁和隔膜中有黑色素存在。当将L3 - 4椎间盘和骨的软组织接种于双相脑心浸液培养基上培养时，分离出卵形瓶霉。菌落湿润，灰白色至赭色，背面有特征性的绿色、可扩散色素。该分离株在40摄氏度时生长良好。它形成特征性的无明显颈环和基部隔膜的瓶梗，并产生光滑、单细胞、透明、倒卵形的分生孢子。
