Lomax L G, Cole J R, Padhye A A, Ajello L, Chandler F W, Smith B R
J Clin Microbiol. 1986 May;23(5):987-91. doi: 10.1128/jcm.23.5.987-991.1986.
Phialemonium obovatum was found to be the cause of osteomyelitis in a German shepherd dog. Histologic examination of the biopsied material from the left tibia revealed septate, irregularly branched hyphae, swollen cells, and ovate-to-spherical cells divided by a transverse septum. The majority of the fungal elements were hyaline, but a few had lightly pigmented cell walls that had a greenish yellow tint. The presence of melanin in the cell walls of the hyphae and especially in their septa was verified by the use of the Fontana-Masson silver stain. P. obovatum formed moist, off-white-to-ochraceous, spreading colonies with a characteristic green pigment on their reverse side. The pigment was more prominent in cultures grown at 37 degrees C than in those grown at 25 degrees C. The isolate also grew at 40 degrees C. The dog isolate produced characteristic adelophialides without conspicuous collarettes and also basal septa from the creeping vegetative hyphae growing on the surface of the medium. The numerous obovate phialoconidia were smooth and one-celled.
卵形瓶霉被发现是一只德国牧羊犬骨髓炎的病因。对左胫骨活检材料的组织学检查显示有分隔、不规则分支的菌丝、肿胀细胞以及被横向隔膜分隔的卵形至球形细胞。大多数真菌成分是透明的，但少数细胞壁有轻微色素沉着，呈黄绿色调。通过使用Fontana-Masson银染法证实了菌丝细胞壁尤其是隔膜中存在黑色素。卵形瓶霉形成湿润的、灰白色至赭色的、蔓延的菌落，其背面有特征性的绿色色素。该色素在37℃培养的菌落中比在25℃培养的菌落中更明显。该分离株也能在40℃生长。从犬分离株在培养基表面生长的匍匐营养菌丝上产生特征性的无明显颈环的瓶梗以及基部隔膜。大量卵形瓶梗孢子光滑且单细胞。
