Philip R, Liggitt D, Philip M, Dazin P, Debs R
Cancer Research Institute, University of California, San Francisco 94143-0128.
J Biol Chem. 1993 Aug 5;268(22):16087-90.
We have demonstrated that a single intraperitoneal injection of cationic liposomes complexed to a chloramphenicol acetyltransferase (CAT) gene expression plasmid can transfect the majority of splenic Thy 1.2+ T lymphocytes, as well as significant numbers of bone marrow-derived hematopoietic cells, in adult mice. CAT activity was detected in the spleen for at least 2 weeks, and there was no evidence of treatment-related toxicity. Some degree of tissue-specific transgene expression was achieved by varying the cationic lipid used.
