Jyothi R, Foerster B, Hamelmann C, Shetty N P
Department of Microbiology, St John's Medical College, Bangalore, India.
J Trop Med Hyg. 1993 Aug;96(4):249-50.
Human stool samples are the most widely used source of E. histolytica cysts for diagnosis and research. Cysts concentrated from faecal samples are contaminated with faecal debris and bacteria and are unsuitable for use as antigens. Other gradient purification and sedimentation procedures result in considerable cyst losses. We present an effective and simple purification procedure using varying densities of Percoll that yields an adequate cyst concentration from a moderate cyst load in a single stool sample.
人类粪便样本是用于诊断和研究的溶组织内阿米巴包囊最广泛使用的来源。从粪便样本中浓缩得到的包囊被粪便残渣和细菌污染，不适宜用作抗原。其他梯度纯化和沉降程序会导致大量包囊损失。我们提出了一种有效且简单的纯化程序，使用不同密度的 Percoll，可从单个粪便样本中的适度包囊负荷中获得足够的包囊浓度。
