Sakai H, Mori K, Katayama Y, Matsuyama T
Department of Clinical Laboratory, National Cardiovascular Center Hospital, Suita.
Rinsho Byori. 1993 Mar;41(3):293-7.
To analyze phagocytosis mechanism of granulocyte using fluorescent microspheres, changes of phagocytic index, complement receptors, Fc receptors and adhesion molecules on cell membrane, after exogenous stimulation were determined by flow cytometry. The changes of phagocytic index by blocking test was also determined using monoclonal antibodies for receptors. The phagocytic index was decreased by supernatant of S. aureus, and was increased by supernatant of E. coli and phorbol myristate acetate (PMA). By PMA stimulation, surface marker positive cells increased in CR1, IgGFcR2 and decreased in IgGFcR3, LFA-3, Homing receptor, whereas they increased in CR1, IgMFcR, LFA-1 beta and decreased in LFA-3 by the supernatants of S. aureus and E. coli. Blocking test using monoclonal antibodies to individual receptors showed decrease of phagocytic index in CR1, CR3, LFA-1 beta. In this phagocytosis using fluorescent microspheres, the complement receptors were most dedicating in phagocytosis of granulocyte.
为利用荧光微球分析粒细胞的吞噬机制，采用流式细胞术检测外源性刺激后吞噬指数、细胞膜上补体受体、Fc受体和黏附分子的变化。还用针对受体的单克隆抗体通过阻断试验测定吞噬指数的变化。金黄色葡萄球菌上清液可降低吞噬指数，而大肠杆菌上清液和佛波酯（PMA）可增加吞噬指数。经PMA刺激后，CR1、IgGFcR2表面标志物阳性细胞增加，IgGFcR3、LFA - 3、归巢受体表面标志物阳性细胞减少；而经金黄色葡萄球菌和大肠杆菌上清液刺激后，CR1、IgMFcR、LFA - 1β表面标志物阳性细胞增加，LFA - 3表面标志物阳性细胞减少。使用针对单个受体的单克隆抗体进行阻断试验显示，CR1、CR3、LFA - 1β的吞噬指数降低。在这种利用荧光微球的吞噬作用中，补体受体在粒细胞吞噬作用中起最主要作用。
