Saito S, Saito M, Enomoto M, Ito A, Motoyoshi K, Nakagawa T, Ichijo M
Department of Obstetrics and Gynecology, Nara Medical University, Japan.
Growth Factors. 1993;9(1):11-9. doi: 10.3109/08977199308991578.
When human cytotrophoblastic cells in the early stage of pregnancy were cultured in a serum-free medium in the presence of human macrophage colony-stimulating factor (M-CSF), the cytotrophoblastic cells fused and formed a typical syncytiotrophoblast which had a dense distribution of microvilli revealed under an electron microscope. On the other hand, cytotrophoblasts incubated with anti-M-CSF antibody showed hardly any syncytiotrophoblast formation. Following this finding, we studied the differentiation of chorionic cells from the viewpoint of hormone secretion. When cytotrophoblasts were incubated in the presence of M-CSF, the supernatant of the culture showed an increase in human chorionic gonadotropin and human placental lactogen levels in proportion to the concentration of M-CSF added. When cytotrophoblasts were incubated in the presence of anti-M-CSF antibody or anti-fms antibody, human chorionic gonadotropin and human placental lactogen secretion were suppressed. Thus, M-CSF was morphologically and endocrinologically found to induce the differentiation of chorionic cells.
当妊娠早期的人细胞滋养层细胞在无血清培养基中、在人巨噬细胞集落刺激因子（M-CSF）存在的情况下培养时，细胞滋养层细胞融合并形成典型的合体滋养层，在电子显微镜下可见其微绒毛分布密集。另一方面，与抗M-CSF抗体孵育的细胞滋养层几乎没有形成合体滋养层。基于这一发现，我们从激素分泌的角度研究了绒毛膜细胞的分化。当细胞滋养层在M-CSF存在的情况下孵育时，培养上清液中人类绒毛膜促性腺激素和人胎盘催乳素水平随添加的M-CSF浓度成比例增加。当细胞滋养层在抗M-CSF抗体或抗fms抗体存在的情况下孵育时，人类绒毛膜促性腺激素和人胎盘催乳素的分泌受到抑制。因此，从形态学和内分泌学角度发现M-CSF可诱导绒毛膜细胞的分化。
