Adkins J P, Madigan M T, Mandelco L, Woese C R, Tanner R S
Department of Botany and Microbiology, University of Oklahoma, Norman 73019.
Int J Syst Bacteriol. 1993 Jul;43(3):514-20. doi: 10.1099/00207713-43-3-514.
Arhodomonas aquaeolei gen. nov., sp. nov., isolated from a petroleum reservoir production fluid, is described. The single isolate was an obligately halophilic, aerobic, gram-negative, oval rod-shaped bacterium that was actively motile by means of a single polar flagellum. It was catalase and oxidase positive. The isolate had a specific requirement for NaCl; growth occurred at NaCl concentrations between 6 and 20%, and optimal growth occurred in the presence of 15% NaCl. This species metabolized primarily organic acids and required biotin for growth. The name Arhodomonas is proposed for the new genus, which was placed in the gamma subclass of the Proteobacteria on the basis of the results of a 16S rRNA sequence analysis. Although A. aquaeolei is most closely related to purple sulfur bacteria (the genera Ectothiorhodospira and Chromatium), it is not a phototrophic microorganism, which is consistent with its isolation from a subterranean environment. The major components of its cellular fatty acids were C16:0, C18:1, C19:0, C16:1, and C18:0 acids. The DNA base composition of the type strain is 67 mol% G+C. The type and only strain is strain HA-1 (= ATCC 49307).
描述了从石油储层采出液中分离出的水生红嗜盐菌属（Arhodomonas）新属、新种。该单一分离株是一种专性嗜盐、需氧、革兰氏阴性、椭圆形杆状细菌，通过单根极生鞭毛进行活跃运动。它过氧化氢酶和氧化酶呈阳性。该分离株对氯化钠有特定需求；在氯化钠浓度为6%至20%时生长，在15%氯化钠存在下生长最佳。该物种主要代谢有机酸，生长需要生物素。提议将Arhodomonas作为新属名，基于16S rRNA序列分析结果，该属被置于变形菌门的γ亚纲。虽然水生红嗜盐菌与紫色硫细菌（外硫红螺菌属和着色菌属）关系最为密切，但它不是光合微生物，这与其从地下环境中分离一致。其细胞脂肪酸的主要成分是C16:0、C18:1、C19:0、C16:1和C18:0酸。模式菌株的DNA碱基组成为67 mol% G+C。模式菌株也是唯一菌株是HA-1菌株（= ATCC 49307）。
