Suzuki T, Dale G L
Blood. 1987 Sep;70(3):791-5.
Rabbit erythrocytes were biotinylated by reaction with N-hydroxysuccinimidobiotin; the average level of biotinylation was 25,000 molecules per erythrocyte. These biotinylated cells exhibited a normal survival rate when reinfused into rabbits. Two studies demonstrated that the biotin label was stable in vivo. The first was a double-labeling experiment where the biotinylated erythrocytes were also labeled with 14C-cyanate; on reinfusion, the loss of biotinylated erythrocytes and 14C-cyanate label occurred in unison. The second study demonstrated that biotinylated erythrocytes that had been reinfused into rabbits could later be selectively isolated by attachment to an avidin support. This technique will facilitate a variety of studies that require the ability to label a specific cohort of cells in vitro and then reisolate those same cells after in vivo recirculation.
兔红细胞通过与N-羟基琥珀酰亚胺生物素反应进行生物素化；平均生物素化水平为每个红细胞25,000个分子。将这些生物素化的细胞回输到兔子体内时，其存活率正常。两项研究表明生物素标记在体内是稳定的。第一项是双标记实验，其中生物素化的红细胞也用14C-氰酸盐标记；回输时，生物素化红细胞和14C-氰酸盐标记的丢失是一致的。第二项研究表明，回输到兔子体内的生物素化红细胞后来可以通过附着于抗生物素蛋白支持物而被选择性分离。这项技术将有助于开展各种研究，这些研究需要具备在体外标记特定细胞群体，然后在体内再循环后重新分离出相同细胞的能力。
