Liu J, Zhang Y, Shen G, Wang X, Su N, Zhu H
Department of Urology, Tongji Hospital, Wuhan, People's Republic of China.
Int J Fertil Menopausal Stud. 1993 May-Jun;38(3):187-91.
To determine subsets and functional characteristics of peripheral blood lymphocytes in men with infertility associated with anti-sperm antibodies.
Blood from 28 infertile men with anti-sperm antibodies and 34 fertile control males were tested for the lymphocyte subsets by indirect immunofluorescence and also for lymphocyte transformation responses to phytohemagglutinin (PHA), ConA, and pokeweed mitogen (PWM). Also studied were effects of seminal plasma on the lymphocyte transformation responses.
Percentages of CD3, -4, and -8 cells and lymphocyte blastogenic responses to PHA and ConA were significantly lowered in patients. Percentage of B-cells, CD4/CD8 ratio, and blastogenic response to PWM were increased. All seminal plasmas suppressed lymphocyte blastogenesis induced by PHA, PWM, and ConA, but the suppressive effect on PHA and PWM by patients' plasma was less than that of controls.
The results suggest that number and function of T-lymphocytes, especially CD8 cells, are lessened and those of CD4 and B-cells enhanced in the infertile patients. These factors would be conducive to development of an immunologic response to sperm.
确定抗精子抗体相关男性不育患者外周血淋巴细胞的亚群及功能特征。
采用间接免疫荧光法检测28例抗精子抗体阳性不育男性及34例生育力正常对照男性的淋巴细胞亚群，并检测其对植物血凝素（PHA）、刀豆蛋白A（ConA）和商陆有丝分裂原（PWM）的淋巴细胞转化反应。同时研究精浆对淋巴细胞转化反应的影响。
患者的CD3、CD4和CD8细胞百分比以及淋巴细胞对PHA和ConA的增殖反应显著降低。B细胞百分比、CD4/CD8比值以及对PWM的增殖反应增加。所有精浆均抑制PHA、PWM和ConA诱导的淋巴细胞增殖，但患者精浆对PHA和PWM的抑制作用小于对照。
结果提示，不育患者T淋巴细胞数量及功能，尤其是CD8细胞减少，而CD4和B细胞功能增强。这些因素有利于对精子产生免疫反应。
