Van Petegem M, Cartuyvels R, de Schouwer P, van Duppen V, Goossens W, van Hove L
University Hospital Leuven, Department of Haematology, Belgium.
Clin Lab Haematol. 1993;15(2):103-11. doi: 10.1111/j.1365-2257.1993.tb00133.x.
Flow cytometric determination of reticulocytosis is progressively replacing the manual microscopic method. We evaluated three flow cytometers (Becton Dickinson FACScan, Coulter EPICS Profile II, Ortho Cytoron Absolute) for reticulocyte enumeration, using thiazole orange. For each sample, 30,000 cells were analysed. In order to comparatively evaluate the three instruments, reticulocytes were counted by manually gating the erythroid population and evaluating the gated population for fluorescence characteristics. The different instruments showed good linearity and precision. No carry-over was observed. Orthogonal regression analysis of reticulocyte counts of 100 healthy blood donor samples and 108 haematological patient samples showed a good mutual comparability between all three instruments tested, although the paired t-test showed a significant difference between the Cytoron and both the FACScan and the Profile. Despite minor statistical differences, the three instruments can be considered equivalent for daily routine reticulocyte enumeration.
流式细胞术检测网织红细胞增多症正在逐步取代手工显微镜检查法。我们使用噻唑橙，评估了三台流式细胞仪（贝克曼库尔特公司的FACScan、库尔特公司的EPICS Profile II、奥多公司的Cytoron Absolute）用于网织红细胞计数。对每个样本分析30,000个细胞。为了比较评估这三台仪器，通过手动圈定红细胞群体并评估圈定群体的荧光特征来计数网织红细胞。不同仪器显示出良好的线性和精密度。未观察到残留。对100份健康献血者样本和108份血液学患者样本的网织红细胞计数进行正交回归分析表明，尽管配对t检验显示Cytoron与FACScan和Profile之间存在显著差异，但所测试的所有三台仪器之间具有良好的相互可比性。尽管存在微小的统计学差异，但这三台仪器在日常常规网织红细胞计数方面可被视为等效。
