Ohhara Y
Third Department of Internal Medicine, Hokkaido University School of Medicine, Sapporo City, Japan.
Eur J Haematol. 1993 Jul;51(1):31-7. doi: 10.1111/j.1600-0609.1993.tb00601.x.
The red cell ferritin (rFt) level in 10 patients with refractory anemia (RA) was measured and analyzed by column isoelectric focusing (IEF). The levels in 9 of the 10 patients (90.0%) were higher than the upper limit in healthy controls (MV +/- SD: male 14.3 +/- 10.3 ag/cell, female 7.5 +/- 3.6 ag/cell). The isoelectric point (pI) of rFt determined by IEF in healthy subjects ranged from 5.1 to 5.7. However, the pI ranges for RA patients varied widely; the pI value was thought to correlate with the severity of the morphological abnormalities of bone marrow (BM) erythroblasts. That is, the greater the proportion of morphologically abnormal erythroblasts with respect to all erythroid precursors, the lower the pI range for rFt. The rFt content was not related to serum iron, transferrin saturation, serum ferritin, reticulocyte count, red cell iron content, or BM erythroblast count. These data suggest that rFt synthesis in RA patients is influenced by factor(s) other than iron; this is considered an essential feature of erythropoiesis in myelodysplastic syndrome (MDS).
采用柱等电聚焦法（IEF）对10例难治性贫血（RA）患者的红细胞铁蛋白（rFt）水平进行了测定和分析。10例患者中有9例（90.0%）的rFt水平高于健康对照的上限（均值±标准差：男性14.3±10.3 ag/细胞，女性7.5±3.6 ag/细胞）。健康受试者经IEF测定的rFt等电点（pI）范围为5.1至5.7。然而，RA患者的pI范围差异很大；pI值被认为与骨髓（BM）成红细胞形态异常的严重程度相关。也就是说，形态异常的成红细胞相对于所有红系前体细胞的比例越高，rFt的pI范围越低。rFt含量与血清铁、转铁蛋白饱和度、血清铁蛋白、网织红细胞计数、红细胞铁含量或BM成红细胞计数无关。这些数据表明，RA患者的rFt合成受铁以外的因素影响；这被认为是骨髓增生异常综合征（MDS）红细胞生成的一个基本特征。
