Chan F T, Guan M X, Mackenzie A M
Department of Laboratory Medicine, Children's Hospital of Eastern Ontario, Ottawa, Canada.
J Clin Microbiol. 1993 Jul;31(7):1710-4. doi: 10.1128/jcm.31.7.1710-1714.1993.
An indirect fluorescent-antibody (IFA) assay was carried out to examine for the presence of Dientamoeba fragilis trophozoites in preserved fecal specimens. Antiserum to D. fragilis trophozoites was raised in a rabbit with a dixenic culture of D. fragilis (ATCC 30948) from the American Type Culture Collection. After absorption with Klebsiella pneumoniae and Bacteroides vulgatus, the immune rabbit serum was used for examination by the IFA assay. A total of 155 clinical samples were tested; 42 with no parasites, 9 with D. fragilis, and 104 with other parasites. The IFA assay identified seven D. fragilis organisms. Two specimens with doubtful IFA assay readings showed very scanty amounts of D. fragilis trophozoites on stained smears. There were no false-positive IFA assay readings. The IFA assay appeared to be a promising method because of its speed in screening. The specificity of the IFA assay indicates that other diagnostic tests such as an enzyme-linked immunosorbent assay could be developed to identify D. fragilis antigens in fecal specimens.
采用间接荧光抗体（IFA）检测法，检查保存粪便标本中脆弱双核阿米巴滋养体的存在情况。用来自美国模式培养物集存库（ATCC 30948）的脆弱双核阿米巴双相培养物在兔体内制备抗脆弱双核阿米巴滋养体的抗血清。用肺炎克雷伯菌和普通拟杆菌吸收后，将免疫兔血清用于IFA检测。共检测了155份临床样本；42份未检出寄生虫，9份检出脆弱双核阿米巴，104份检出其他寄生虫。IFA检测法鉴定出7个脆弱双核阿米巴生物体。两份IFA检测结果存疑的标本，在染色涂片上显示脆弱双核阿米巴滋养体数量极少。IFA检测法无假阳性结果。由于其筛查速度快，IFA检测法似乎是一种很有前景的方法。IFA检测法的特异性表明，可以开发其他诊断试验，如酶联免疫吸附测定法，以鉴定粪便标本中的脆弱双核阿米巴抗原。
