用于确定汉赛巴尔通体生长曲线和优化其生长的无血培养基的研发与评估
Development and evaluation of a blood-free medium for determining growth curves and optimizing growth of Rochalimaea henselae.
作者信息
Schwartzman W A, Nesbit C A, Baron E J
机构信息
Department of Medicine, University of California, Los Angeles 90024.
出版信息
J Clin Microbiol. 1993 Jul;31(7):1882-5. doi: 10.1128/jcm.31.7.1882-1885.1993.
Abstract
Two strains of Rochalimaea henselae were used to optimize a blood-free growth medium. Seven agar bases, four broths, and combinations of eight supplements were evaluated. Acceptable growth was achieved in media containing Fildes solution and hemin, with the best growth demonstrated in brucella broth or on brucella agar with 6 to 8% Fildes solution and 250 micrograms of hemin per ml. R. henselae utilized hemin in concentrations six times that utilized by Rochalimaea quintana. Erythrocyte membrane was necessary to achieve the full growth-promoting effect of rabbit blood.
摘要
使用两株汉赛罗卡利马氏体来优化无血生长培养基。评估了七种琼脂基础培养基、四种肉汤培养基以及八种补充剂的组合。在含有菲尔德氏溶液和血红素的培养基中实现了可接受的生长，在含有6%至8%菲尔德氏溶液和每毫升250微克血红素的布鲁氏菌肉汤或布鲁氏菌琼脂上生长最佳。汉赛罗卡利马氏体利用血红素的浓度是五日罗卡利马氏体的六倍。红细胞膜对于实现兔血的完全促生长作用是必要的。
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Rochalimaea henselae sp. nov., a cause of septicemia, bacillary angiomatosis, and parenchymal bacillary peliosis.新种汉赛巴尔通体，一种引起败血症、杆菌性血管瘤病和实质性杆菌性紫癜的病原体。
J Clin Microbiol. 1992 Feb;30(2):275-80. doi: 10.1128/jcm.30.2.275-280.1992.
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