Chow E, Wirtz R A, Scott T W
Department of Entomology, University of Maryland, College Park 20742.
J Am Mosq Control Assoc. 1993 Jun;9(2):196-205.
A comparative study of 3 ELISA protocols was performed for host blood meal identification in Aedes aegypti. In the sandwich-B ELISA, which used a combination of heavy and heavy+light chain conjugates, specificity was improved to such a degree that conjugates no longer required cross-adsorption with heterologous sera. Using the sandwich-B assay, human blood meals in laboratory reared Ae. aegypti could be detected longer after feeding (100% at 32 h and 80% at 42 h) than with a direct assay (100% at 20 h). Efficacy of the sandwich-B and direct ELISAs in field analyses was studied in parallel) using 80 field collected mosquitoes from Thailand. The sandwich-B assay was superior (88% detection rate) to the direct assay (41% detection rate) and was thus selected as the method of choice for future field studies.
为了鉴定埃及伊蚊的宿主血餐，对3种酶联免疫吸附测定（ELISA）方案进行了比较研究。在使用重链和重链+轻链缀合物组合的夹心-B ELISA中，特异性提高到了这样的程度，即缀合物不再需要与异源血清进行交叉吸附。使用夹心-B检测法，实验室饲养的埃及伊蚊在进食后更长时间仍可检测到人类血餐（32小时时为100%，42小时时为80%），而直接检测法（20小时时为100%）则不然。同时使用从泰国采集的80只野外蚊子研究了夹心-B ELISA和直接ELISA在野外分析中的效果。夹心-B检测法（检测率88%）优于直接检测法（检测率41%），因此被选为未来野外研究的首选方法。
