利什曼原虫RNA病毒1蛋白的检测

Cadd T L, Keenan M C, Patterson J L

Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115.

J Virol. 1993 Sep;67(9):5647-50. doi: 10.1128/JVI.67.9.5647-5650.1993.

Polyclonal antiserum was raised against the peak viral fraction of a sucrose gradient from LRV1-4-infected cells and used in Western immunoblot analysis to identify viral proteins from various isolates. Consistent with this result, in vitro-translated protein from cloned RNA was immunoprecipitated with the same antiserum. The putative capsid at times appeared as a doublet; relative amounts of the two species varied, depending on the method of purification.

针对来自感染LRV1 - 4细胞的蔗糖梯度中的病毒峰值组分制备了多克隆抗血清，并用于蛋白质免疫印迹分析，以鉴定来自各种分离株的病毒蛋白。与该结果一致，来自克隆RNA的体外翻译蛋白也能用相同的抗血清进行免疫沉淀。假定的衣壳有时表现为双重条带；这两种形式的相对含量有所不同，具体取决于纯化方法。