Massie H R, Aiello V R, Williams T R
Masonic Medical Research Laboratory, Utica, NY 13501.
Mech Ageing Dev. 1993 May;68(1-3):175-82. doi: 10.1016/0047-6374(93)90149-l.
The life span of adult Drosophila melanogaster fruit flies changed when they were fed two different photosensitizers. Methylene blue decreased the median life span by 49% when present in the food at a concentration of 0.001 M. Another photosensitizer, riboflavin, produced no changes in life span under the same conditions of a 12:12 h light/dark cycle at a daytime light intensity of 1000 lux. Flies exposed to constant darkness lived 43.2% longer than those exposed to constant light at a light intensity of 2000 lux. Under these conditions, riboflavin increased the life span of the flies exposed to constant light by as much as 25%. We conclude that riboflavin confers some degree of protection against the effects of constant light exposure. The completely different results obtained with riboflavin and methylene blue suggest a possible mechanism for photoageing involving photodynamic action mediated through the production of singlet oxygen.
当成年黑腹果蝇被喂食两种不同的光敏剂时，它们的寿命发生了变化。当亚甲蓝以0.001 M的浓度存在于食物中时，其将果蝇的平均寿命缩短了49%。另一种光敏剂核黄素，在12:12小时光照/黑暗周期、白天光照强度为1000勒克斯的相同条件下，对果蝇寿命没有影响。暴露于持续黑暗中的果蝇比暴露于光照强度为2000勒克斯的持续光照下的果蝇寿命长43.2%。在这些条件下，核黄素使暴露于持续光照下的果蝇寿命延长了25%。我们得出结论，核黄素对持续光照的影响具有一定程度的保护作用。核黄素和亚甲蓝得到的完全不同的结果表明，光老化可能存在一种涉及通过单线态氧产生介导的光动力作用的机制。
