Kurtin P J, Roche P C
Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota 55905.
Am J Surg Pathol. 1993 Sep;17(9):898-904. doi: 10.1097/00000478-199309000-00005.
Paraffin sections of 133 cases of non-Hodgkin's lymphoma (69 B-cell type and 64 T-cell type) were stained in a labeled streptavidin biotin immunoperoxidase technique with a panel of antibodies that recognized T-lymphocyte associated antigens. This study was done to determine the sensitivity and specificity of these reagents for phenotyping T-cell lymphomas. UCHL-1, polyclonal anti-CD3, Leu-22, and OPD4 stained 78%, 72%, 91%, and 69% of the cases of T-cell lymphomas, respectively. The phenotype of L-26 negative and CD3 or UCHL-1 positive accurately predicted T-cell phenotype in 95% (60 of 63) of the T-cell lymphomas and was not seen in any of the cases of B-cell lymphoma. Although Leu-22 was the most sensitive T-cell-associated marker in this series, its lack of specificity for T-lymphocytes limited its usefulness as part of a routine panel designed to distinguish between T-cell and B-cell lymphomas. In conjunction with other reports, this study supports the use of the T-cell markers CD3 and UCHL-1 in combination with the B-cell-associated marker L-26 to phenotype most efficiently non-Hodgkin's lymphomas in paraffin sections.
对133例非霍奇金淋巴瘤（69例B细胞型和64例T细胞型）的石蜡切片，采用标记链霉亲和素生物素免疫过氧化物酶技术，用一组识别T淋巴细胞相关抗原的抗体进行染色。本研究旨在确定这些试剂对T细胞淋巴瘤进行表型分析的敏感性和特异性。UCHL-1、多克隆抗CD3、Leu-22和OPD4分别对78%、72%、91%和69%的T细胞淋巴瘤病例染色。L-26阴性且CD3或UCHL-1阳性的表型在95%（63例中的60例）的T细胞淋巴瘤中准确预测了T细胞表型，而在任何B细胞淋巴瘤病例中均未出现。尽管Leu-22是本系列中最敏感的T细胞相关标志物，但其对T淋巴细胞缺乏特异性，限制了其作为区分T细胞和B细胞淋巴瘤的常规检测组合一部分的用途。结合其他报告，本研究支持使用T细胞标志物CD3和UCHL-1与B细胞相关标志物L-26联合，以最有效地对石蜡切片中的非霍奇金淋巴瘤进行表型分析。
