Salisbury J R, Rapson N T, Codd J D, Rogers M V, Nethersell A B
Department of Histopathology, King's College School of Medicine and Dentistry, London.
J Clin Pathol. 1994 Apr;47(4):313-7. doi: 10.1136/jcp.47.4.313.
To determine the antigen expression of CDw52 using Campath-1 antibodies in a series of non-Hodgkin's lymphomas (NHLs).
Tissue sections of lymphoma were stained immunohistochemically using rat Campath-1G and humanised Campath-1H with avidin-biotin-peroxidase complex techniques. Fifty-two fresh frozen lymphomas and a further 26 paraffin wax embedded sections were studied.
Thirty-seven out of 41 B cell lymphomas were positive with Campath-1H in frozen sections (low grade, 24 of 24; high grade, 13 of 17) as were three out of five T cell lymphomas. Reed-Sternberg cells in six cases of Hodgkin's disease did not react. Eleven out of 16 high grade B cell lymphomas also stained positively with Campath-1G in paraffin wax sections as did five out of 10 T cell lymphomas.
The Campath-1 antibodies showed that CDw52 antigen expression was present in all cases of low grade B cell NHL examined. Immunohistochemical staining in high grade B cell NHL and in T cell NHL was variable. These findings may be relevant to patient selection when considering treatment with Campath-1 antibodies.
使用Campath-1抗体确定一系列非霍奇金淋巴瘤（NHL）中CDw52的抗原表达。
采用抗生物素蛋白-生物素-过氧化物酶复合物技术，用大鼠Campath-1G和人源化Campath-1H对淋巴瘤组织切片进行免疫组织化学染色。研究了52例新鲜冰冻淋巴瘤以及另外26例石蜡包埋切片。
41例B细胞淋巴瘤中有37例在冰冻切片中Campath-1H呈阳性（低级别，24例中的24例；高级别，17例中的13例），5例T细胞淋巴瘤中有3例呈阳性。6例霍奇金病中的里德-斯腾伯格细胞无反应。16例高级别B细胞淋巴瘤中有11例在石蜡切片中Campath-1G也呈阳性，10例T细胞淋巴瘤中有5例呈阳性。
Campath-1抗体显示，在所检查的所有低级别B细胞NHL病例中均存在CDw52抗原表达。高级别B细胞NHL和T细胞NHL中的免疫组织化学染色情况不一。这些发现可能与考虑使用Campath-1抗体进行治疗时的患者选择有关。
