睾丸内注射抗促肾上腺皮质激素释放因子抗血清（a-CRF）对新生大鼠睾丸功能的影响。

Effect of intratesticular administration of anti-corticotropin-releasing factor antiserum (a-CRF) on testicular function in neonatal rats.

作者信息

Gerendai I, Nemeskéri A, Csernus V

机构信息

2nd Department of Anatomy, Semmelweis University Medical School, Budapest, Hungary.

出版信息

Andrologia. 1993 Jul-Aug;25(4):211-5. doi: 10.1111/j.1439-0272.1993.tb02711.x.

DOI:10.1111/j.1439-0272.1993.tb02711.x

PMID:8352430

Abstract

The possible physiological role of testicular corticotropin-releasing factor (CRF) in the regulation of testicular functions was studied in neonatal rats. Two microlitres of anti-CRF-antiserum (dilution: 1:10 or 1:100) was injected intratesticularly to 5 d-old rats with two testes and to hemicastrates. Five days after hemicastration and treatment of the remaining testis with the antiserum, serum testosterone concentration and basal testosterone secretion in vitro decreased significantly. Unilateral testicular injection of a-CRF in rats with two testes resulted in a significant drop in serum testosterone level with no change in basal testosterone production. Data indicate that in neonatal rats testicular CRF might be a local stimulator of steroidogenesis.

摘要

在新生大鼠中研究了睾丸促肾上腺皮质激素释放因子（CRF）在调节睾丸功能方面可能的生理作用。将两微升抗CRF抗血清（稀释度：1:10或1:100）睾丸内注射到具有两个睾丸的5日龄大鼠以及半阉割大鼠体内。半阉割并用抗血清处理剩余睾丸5天后，血清睾酮浓度和体外基础睾酮分泌显著降低。对具有两个睾丸的大鼠单侧睾丸注射α-CRF导致血清睾酮水平显著下降，而基础睾酮生成无变化。数据表明，在新生大鼠中，睾丸CRF可能是类固醇生成的局部刺激物。