Gerendai I, Csaba Z, Csernus V
2nd Department of Anatomy, Semmelweis University Medical School, Budapest, Hungary.
Neuroendocrinology. 1996 Mar;63(3):284-9. doi: 10.1159/000126967.
Previous studies indicated that in immature rats testicular administration of oxytocin stimulates testicular steroidogenesis. In the present study, testicular treatment with oxytocin (50 ng) was combined with pharmacological or surgical denervation of the testis in hemigonadectomized immature rats. For denervation 5,6-dihydroxytryptamine (160 micrograms/testis), a substance that destroys serotoninergic neuronal elements, was injected intratesticularly or vasectomy was performed, which also includes transection of the inferior testicular nerve. In 9-day-old animals both vasectomy and pretreatment of the testis with 5,6-dihydroxytryptamine prevented the oxytocin-induced rise in serum testosterone concentration. In addition, intratesticular injection of oxytocin combined with vasectomy resulted in a significant increase in in vitro basal testosterone secretion of the testis. A similar effect was not observed in the 5,6-dihydroxytryptamine-pretreated group receiving oxytocin. The results indicate that testicular innervation is involved in the control of local peptide effects, and data further suggest a differential role of these neural elements in intratesticular regulatory processes.
先前的研究表明，在未成熟大鼠中，睾丸内给予催产素可刺激睾丸类固醇生成。在本研究中，对半侧去势未成熟大鼠的睾丸进行催产素（50纳克）处理，并结合药理学或手术去神经支配。为了去神经支配，将5,6 - 二羟基色胺（160微克/睾丸），一种破坏血清素能神经元成分的物质，经睾丸内注射，或进行输精管切除术，这也包括切断睾丸下神经。在9日龄动物中，输精管切除术和用5,6 - 二羟基色胺对睾丸进行预处理均阻止了催产素诱导的血清睾酮浓度升高。此外，睾丸内注射催产素并结合输精管切除术导致睾丸体外基础睾酮分泌显著增加。在接受催产素的5,6 - 二羟基色胺预处理组中未观察到类似效果。结果表明，睾丸神经支配参与局部肽效应的控制，并且数据进一步表明这些神经元在睾丸内调节过程中具有不同作用。
