Smirnov V V, Osadchaia A I, Kudriavtsev V A, Safronova L A
Mikrobiol Zh (1978). 1993 May-Jun;55(3):38-44.
The dynamics of accumulation of Bacillus subtilis 39 cells and spores under different conditions of aeration has been studied. It is shown possible to intensify the process of growth, biomass accumulation and sporulation of bacteria using aeration conditions. The growth of cells of the studied bacilli proceeds under conditions of providing the culture with oxygen according to its real demands for the whole period of fermentation. The mathematical models have been obtained which connect the aeration conditions with the rate of biomass accumulation and sporulation of the studied bacteria. It has been stated that with an increase of aeration the yield of microbic cells, degree of using the substrate by them as well as the specific growth rate become higher. Intensive aeration 4.4 = 5.8 g O2/l.h promotes the creation of the most optimal conditions for the growth of bacteria. While deteriorating the aeration to 3.2 g O2/l.h and over, the specific growth rate and yield of viable cells decrease. The optimal conditions of air feed and medium agitation rate decrease the duration of fermentation exerting no effect on the final yield of biomass. It is shown that yield of biomass and spores depends on the growth phase.
研究了不同通气条件下枯草芽孢杆菌39细胞和芽孢的积累动态。结果表明，利用通气条件可以强化细菌的生长、生物量积累和芽孢形成过程。所研究芽孢杆菌的细胞生长在整个发酵期间根据培养物对氧气的实际需求为其提供氧气的条件下进行。已获得将通气条件与所研究细菌的生物量积累速率和芽孢形成速率联系起来的数学模型。研究表明，随着通气量的增加，微生物细胞产量、它们对底物的利用程度以及比生长速率都会提高。强烈通气4.4 = 5.8克氧气/升·小时促进了细菌生长最适宜条件的形成。当通气量恶化至3.2克氧气/升·小时及更低时，比生长速率和活细胞产量会下降。空气供给和培养基搅拌速率的最佳条件可缩短发酵持续时间，而对生物量的最终产量没有影响。结果表明，生物量和芽孢的产量取决于生长阶段。
