Shaari C M, Sanders I
Department of Otolaryngology-Head and Neck Surgery, Mount Sinai Medical Center, New York, NY 10029-6574.
Muscle Nerve. 1993 Sep;16(9):964-9. doi: 10.1002/mus.880160913.
Despite the widespread use of botulinum toxin to treat muscle dystonias, no method exists to quantify muscle paralysis in either human or nonhuman models. In this study we examined how the location, dose, and volume of botulinum injection affects paralysis in the rat tibialis anterior muscle. Paralysis was quantified by electrically stimulating the nerve to the tibialis anterior and then staining sections of the muscle for glycogen. The areas of glycogen-containing fibers represented regions of botulinum action. The results showed that the most important injection technique is to inject botulinum directly into the motor endplate region of a muscle. Injections only 0.5 cm from the motor endplate resulted in a 50% decrease in paralysis. Increases in dose increased paralysis, however, some of that increase was simply due to the increased volume of injection. Thus, delivering toxin in small volumes near the MEP band of a muscle should produce the most effective paralysis.
尽管肉毒杆菌毒素在治疗肌肉张力障碍方面被广泛应用，但目前尚无方法可在人类或非人类模型中量化肌肉麻痹程度。在本研究中，我们探究了肉毒杆菌注射的位置、剂量和体积如何影响大鼠胫前肌的麻痹情况。通过电刺激胫前肌的神经，然后对肌肉切片进行糖原染色来量化麻痹程度。含有糖原的纤维区域代表肉毒杆菌的作用区域。结果表明，最重要的注射技术是将肉毒杆菌直接注射到肌肉的运动终板区域。距离运动终板仅0.5厘米处进行注射会导致麻痹程度降低50%。剂量增加会使麻痹程度增加，然而，这种增加部分仅仅是由于注射体积的增加。因此，在肌肉运动终板带附近以小体积注射毒素应能产生最有效的麻痹效果。
